Immunohistochemistry (IHC)
(Immunochemical staining of human TFRC in human placenta with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.)
Rabbit anti-Human Transferrin Receptor/TFRC Polyclonal Antibody | anti-TFRC antibody
Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IP: 1-4uL/mg of lysate
Immunohistochemistry (IHC)
(Immunochemical staining of human TFRC in human placenta with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.)
Immunohistochemistry (IHC)
(Immunochemical staining of human TFRC in human placenta with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.)
Immunohistochemistry (IHC)
(Immunochemical staining of human TFRC in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.)
Immunohistochemistry (IHC)
(Immunochemical staining of human TFRC in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.)
Immunohistochemistry (IHC)
(Immunochemical staining of human TFRC in human colon carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to intestinal gland.)
Immunohistochemistry (IHC)
(Immunochemical staining of human TFRC in human colon carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to intestinal gland.)
Western Blot (WB)
(Anti-TFRC rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:98 kDaObserved band size:80 kDa)
Western Blot (WB)
(Anti-TFRC rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:98 kDaObserved band size:80 kDa)
Immunoprecipitation (IP)
(TFRC was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg K562 Whole Cell LysateLane C:0.5 mg 293T Whole Cell LysateLane D:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-TFRC rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TFRC rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 84 kDaObserved band size: 100 kDa)
Immunoprecipitation (IP)
(TFRC was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg K562 Whole Cell LysateLane C:0.5 mg 293T Whole Cell LysateLane D:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-TFRC rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TFRC rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 84 kDaObserved band size: 100 kDa)
NCBI and Uniprot Product Information
Uniprot Description
TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system.
Protein type: Membrane protein, integral; Receptor, misc.; Cell surface
Chromosomal Location of Human Ortholog: 3q29
Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; vesicle; endosome; external side of plasma membrane
Molecular Function: identical protein binding; protein binding; transferrin receptor activity; double-stranded RNA binding
Biological Process: receptor-mediated endocytosis; viral reproduction; cellular iron ion homeostasis; positive regulation of bone resorption; transferrin transport; osteoclast differentiation; regulation of cell growth; transmembrane transport; regulation of cell proliferation
Research Articles on TFRC
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Product Notes
The TFRC tfrc (Catalog #AAA8104601) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Transferrin Receptor/TFRC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the TFRC tfrc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transferrin Receptor/TFRC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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