Rabbit anti-Human NR1D2 Polyclonal Antibody | anti-NR1D2 antibody

Anti-NR1D2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104180

• Gene Names: NR1D2; RVR; BD73; EAR-1R
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: NR1D2; Polyclonal Antibody; Anti-NR1D2 Antibody; Rabbit Polyclonal; NR1D2 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-NR1D2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NR1D2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NR1D2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human NR1D2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human NR1D2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NR1D2 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:65 kDa Observed band size:60 kDa)

Related Product Information for anti-NR1D2 antibody

Nuclear receptor NR1D2 is originally characterized as the repressor of genes involved in circadian rhythm. NR1D2 was abundant in human glioblastoma (GBM) tissue and cell lines but not primary human astrocytes. Silencing of NR1D2 changed the morphology of GBM cells, inhibited cell proliferation and motility, whereas had no effects on apoptosis. NR1D2 had more targets other than AXL to regulate epithelial-to-mesenchymal transition and cell motility in GBM cells.

NCBI and Uniprot Product Information

• NCBI GI #: 224177491
• NCBI GeneID: 9975
• NCBI Accession #: NP_005117.3
• NCBI GenBank Nucleotide #: NM_005126.4
• UniProt Accession #: Q14995; O00402; Q86XD4; B2R8Q3
• Molecular Weight: 64,625 Da
• NCBI Official Full Name: nuclear receptor subfamily 1 group D member 2 isoform 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group D, member 2
• NCBI Official Symbol: NR1D2
• NCBI Official Synonym Symbols: RVR; BD73; EAR-1R
• NCBI Protein Information: nuclear receptor subfamily 1 group D member 2; rev-erb-beta; rev-erb alpha-related receptor; rev-erba-alpha-related receptor; V-erbA-related protein 1-related; orphan nuclear hormone receptor BD73; nuclear receptor Rev-ErbA beta variant 1; nuclear recepto
• UniProt Protein Name: Nuclear receptor subfamily 1 group D member 2
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR1D2
• UniProt Synonym Gene Names: RVR; EAR-1R
• UniProt Entry Name: NR1D2_HUMAN

NCBI Description

This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]

Uniprot Description

NR1D2: Binds to the sequences 5'-AATGTAGGTCA-3' and 5'- ATAACTAGGTCA-3'. Acts as a potent competitive repressor of ROR alpha function. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 3p24.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; zinc ion binding; steroid hormone receptor activity

Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; regulation of lipid metabolic process; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; rhythmic process; lipid homeostasis; gene expression; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm