Rabbit anti-Cynomolgus PD-1 Polyclonal Antibody | anti-PDCD1 antibody

Anti-PD-1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104177

• Reactivity: Cynomolgus
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PD-1; Polyclonal Antibody; Anti-PD-1 Antibody; Rabbit Polyclonal; PD-1/PDCD1 Antibody; Rabbit PAb; Antigen Affinity Purified; programmed cell death 1; anti-PDCD1 antibody

• Host: Rabbit
• Reactivity: Cynomolgus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Cynomolgus PD-1/PDCD1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PDCD1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Cynomolgus PD-1/PDCD1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Cynomolgus PD-1/PDCD1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PDCD1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:35 kDa)

Immunoprecipitation (IP)

(GATA4 was immunoprecipitated using: Lane A:0.5 mg HEK293 Whole Cell Lysate 4 uL anti-GATA4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-GATA4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa Observed band size :46 kDa)

Related Product Information for anti-PDCD1 antibody

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-gamma by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

Product Categories/Family for anti-PDCD1 antibody

ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

References

James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.

NCBI and Uniprot Product Information

• NCBI GI #: 545688712
• NCBI GeneID: 102123659
• NCBI Accession #: NP_001271065.1
• NCBI GenBank Nucleotide #: NP_001271065.1
• UniProt Accession #: B0LAJ3
• Molecular Weight: Molecular Mass: 17.3kDa; Actual Protein Molecular Mass: 30-50kDa
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Symbol: PDCD1
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death 1
• Protein Family: Programmed cell death protein