Rabbit GABARAP Polyclonal Antibody | anti-GABARAP antibody

Anti-GABARAP Antibody, Rabbit Polyclonal

Cat. Num. AAA8103658

• Gene Names: GABARAP; MM46; ATG8A; GABARAP-a
• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovin, Rabbit (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: GABARAP; Polyclonal Antibody; Anti-GABARAP Antibody; Rabbit Polyclonal; GABARAP Antibody; Rabbit PAb; Antigen Affinity Purified; GABA(A) receptor-associated protein; Anti-ATG8A Antibody; Anti-GABARAP-a Antibody; Anti-MM46 Antibody; anti-GABARAP antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovin, Rabbit (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GABARAP
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GABARAP antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human GABARAP
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human GABARAP, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-GABARAP rabbit polyclonal antibody at 1:500 dilution Lane A: SH-SY5Y Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:14 kDa)

Related Product Information for anti-GABARAP antibody

The Atg8/LC3/GABARAP family of proteins, a group that has structural homology with ubiquitin, connects with a large set of binding partners to function in macroautophagy (hereafter autophagy). GABARAP in tumorigenesis in vivo by delaying cell death and its associated immune-related response. GABARAPs are uniquely required for antimicrobial host defense through cytosolic distribution of interferon-inducible GTPases. GABARAPs as the first known direct interaction partners of Nef that are essential for its plasma membrane localization.

NCBI and Uniprot Product Information

• NCBI GI #: 6005764
• NCBI GeneID: 11337
• NCBI Accession #: NP_009209.1
• NCBI GenBank Nucleotide #: NM_007278.1
• UniProt Accession #: O95166
• Molecular Weight: 13,918 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor-associated protein
• NCBI Official Synonym Full Names: GABA(A) receptor-associated protein
• NCBI Official Symbol: GABARAP
• NCBI Official Synonym Symbols: MM46; ATG8A; GABARAP-a
• NCBI Protein Information: gamma-aminobutyric acid receptor-associated protein
• UniProt Protein Name: Gamma-aminobutyric acid receptor-associated protein
• Protein Family: Gamma-aminobutyric acid receptor-associated protein
• UniProt Gene Name: GABARAP
• UniProt Synonym Gene Names: FLC3B
• UniProt Entry Name: GBRAP_HUMAN

NCBI Description

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008]

Uniprot Description

GABARAP: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Belongs to the MAP1 LC3 family. Interacts with GPHN and NSF. Interacts with GABRG2, beta-tubulin and ULK1.

Protein type: Autophagy; Ubiquitin-like modifier; Adaptor/scaffold; Microtubule-binding; Vesicle

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: microtubule; smooth endoplasmic reticulum; lysosome; autophagic vacuole; cytosol; actin cytoskeleton; Golgi membrane; microtubule associated complex; extrinsic to membrane; perinuclear region of cytoplasm; plasma membrane; axoneme; cytoplasmic vesicle

Molecular Function: protein binding; microtubule binding; beta-tubulin binding; GABA receptor binding

Biological Process: synaptic transmission; induction of apoptosis via death domain receptors; mitochondrion degradation; microtubule cytoskeleton organization and biogenesis; protein targeting; autophagic vacuole formation