Western Blot (WB)
(Anti-PRAP1 rabbit polyclonal antibody at 1:500 dilution Lane A: THP-1 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:19 kDa)
Rabbit anti-Human PRAP1 Polyclonal Antibody | anti-PRAP1 antibody
Anti-PRAP1 Antibody, Rabbit Polyclonal
Gene Names
PRAP1; UPA; PRO1195
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PRAP1; Polyclonal Antibody; Anti-PRAP1 Antibody; Rabbit Polyclonal; PRAP1/Proline-rich acidic protein 1 Antibody; Rabbit PAb; Antigen Affinity Purified; proline-rich acidic protein 1; Anti-PRO1195 Antibody; Anti-UPA Antibody; anti-PRAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PRAP1/Proline-rich acidic protein 1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PRAP1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the center region of the Human PRAP1/Proline-rich acidic protein 1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PRAP1/Proline-rich acidic protein 1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PRAP1 rabbit polyclonal antibody at 1:500 dilution Lane A: THP-1 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:19 kDa)
Western Blot (WB)
(Anti-PRAP1 rabbit polyclonal antibody at 1:500 dilution Lane A: THP-1 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:19 kDa)
Related Product Information for anti-PRAP1 antibody
PRAP1 is a protein interacting partner of MAD1 and that PRAP1 is able to down-regulate MAD1 and suppress mitotic checkpoint signalling in HCC. PRAP1 is a novel p53 target gene. The induction of PRAP1 expression by p53 may promote resistance of cancer cells to chemotherapeutic drugs such as 5-fluorouracil (5-FU), as knockdown of PRAP1 increases apoptosis in cancer cells after 5-FU treatment. PRAP1 appears to protect cells from apoptosis by inducing cell-cycle arrest, suggesting that the induction of PRAP1 expression by p53 in response to DNA-damaging agents contributes to cancer cell survival.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,332 Da
NCBI Official Full Name
proline-rich acidic protein 1 isoform 2
NCBI Official Synonym Full Names
proline-rich acidic protein 1
NCBI Official Symbol
PRAP1
NCBI Official Synonym Symbols
UPA; PRO1195
NCBI Protein Information
proline-rich acidic protein 1; epididymis tissue protein Li 178
UniProt Protein Name
Proline-rich acidic protein 1
Protein Family
UniProt Gene Name
PRAP1
UniProt Synonym Gene Names
UPA
UniProt Entry Name
PRAP1_HUMAN
Uniprot Description
PRAP1: May play an important role in maintaining normal growth homeostasis in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 10q26.3
Cellular Component: extracellular region
Research Articles on PRAP1
Similar Products
Product Notes
The PRAP1 prap1 (Catalog #AAA8103398) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRAP1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PRAP1 prap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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