Western Blot (WB)
(Anti-IL6 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human IL-6 Polyclonal Antibody | anti-IL6 antibody
Anti-IL-6 Antibody, Rabbit Polyclonal
Gene Names
IL6; HGF; HSF; BSF2; IL-6; IFNB2
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
IL-6; Polyclonal Antibody; Anti-IL-6 Antibody; Rabbit Polyclonal; IL6/IL-6 Antibody; Rabbit PAb; Antigen Affinity Purified; interleukin 6; Anti-BSF2 Antibody; Anti-HGF Antibody; Anti-HSF Antibody; Anti-IFNB2 Antibody; Anti-Interleukin-6 Antibody; anti-IL6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human IL6/IL-6
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-IL6 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
IP: 1-2uL/mg of lysate
ELISA: 1:25,000-1:50,000
IP: 1-2uL/mg of lysate
Immunogen
Recombinant Human IL6/IL-6 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human IL6/IL-6 (rh IL6/IL-6). IL6/IL-6 specific IgG was purified by Human IL6/IL-6 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-IL6 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-IL6 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(IL6 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate0.5 uL anti-IL6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-IL6 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)
Immunoprecipitation (IP)
(IL6 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate0.5 uL anti-IL6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-IL6 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)
Related Product Information for anti-IL6 antibody
Interleukin-6 (IL-6) is a multifunctional alpha-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.
Product Categories/Family for anti-IL6 antibody
References
Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
212
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6 (interferon, beta 2)
NCBI Official Symbol
IL6
NCBI Official Synonym Symbols
HGF; HSF; BSF2; IL-6; IFNB2
NCBI Protein Information
interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor
UniProt Protein Name
Interleukin-6
Protein Family
UniProt Gene Name
IL6
UniProt Synonym Gene Names
IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
UniProt Entry Name
IL6_HUMAN
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Product Notes
The IL6 il6 (Catalog #AAA8102639) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-6 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IP: 1-2uL/mg of lysate. Researchers should empirically determine the suitability of the IL6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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