Rabbit anti-Human Occludin Polyclonal Antibody | anti-OCLN antibody

Anti-Occludin Antibody, Rabbit Polyclonal

Cat. Num. AAA8101870

• Gene Names: OCLN; BLCPMG
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: Occludin; Polyclonal Antibody; Anti-Occludin Antibody; Rabbit Polyclonal; OCLN/Occludin Antibody; Rabbit PAb; Antigen Affinity Purified; occludin; Anti-BLCPMG Antibody; Anti-PPP1R115 Antibody; anti-OCLN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human OCLN/Occludin
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-OCLN antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1000-1:5000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human OCLN/Occludin
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human OCLN/Occludin, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-OCLN rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:59 kDa Observed band size:72 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of OCLN in Hek293 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human OCLN polyclonal antibody (dilution ratio 1:1500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane and cytoplasm.)

Related Product Information for anti-OCLN antibody

OCLN (Occludin) is a Protein Coding gene. This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. It belongs to the ELL/occludin family. OCLN is an important component of the tight junction complex, providing apical intercellular connections between adjacent cells in endothelial and epithelial tissue. OCLN, an integral tight junction protein, is one of the key factors for HCV entry into cells. OCLN, a key receptor for HCV, is a candidate target of miR-122; the most abundant hepatic micro RNA. Over-expression of miR-122 can decrease HCV entry into hepatocytes through down-regulation of OCLN. Diseases associated with OCLN include Pseudo-Torch Syndrome 1 and Torch Syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 327478416
• NCBI GeneID: 100506658
• NCBI Accession #: NP_001192184.1
• NCBI GenBank Nucleotide #: NM_001205255.1
• UniProt Accession #: Q16625; Q5U1V4; Q8N6K1; B5BU70; D2DU64; D2DU65; D2IGC0; D2IGC1; E2CYV9
• Molecular Weight: 59,144 Da
• NCBI Official Full Name: occludin isoform b
• NCBI Official Synonym Full Names: occludin
• NCBI Official Symbol: OCLN
• NCBI Official Synonym Symbols: BLCPMG
• NCBI Protein Information: occludin; tight junction protein occludin
• UniProt Protein Name: Occludin
• Protein Family: Occludin
• UniProt Gene Name: OCLN
• UniProt Entry Name: OCLN_HUMAN

NCBI Description

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]

Uniprot Description

occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: tight junction; apicolateral plasma membrane; endocytic vesicle; apical plasma membrane; integral to membrane; plasma membrane; intercellular junction; cytoplasmic vesicle; cell junction; cytosol

Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity

Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; protein complex assembly; cell structure disassembly during apoptosis

Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria