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Western Blot (WB) (Anti-KEL rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:83 kDaObserved band size:85 kDa(We are unsure as to the identity of these extra bands.))

Rabbit anti-Human Kell/KEL Polyclonal Antibody | anti-KEL antibody

Anti-Kell/KEL Antibody, Rabbit Polyclonal

Gene Names
KEL; ECE3; CD238
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Kell/KEL; Polyclonal Antibody; Anti-Kell/KEL Antibody; Rabbit Polyclonal; KEL Antibody; Rabbit PAb; Antigen Affinity Purified; Kell blood group; metallo-endopeptidase; Anti-CD238 Antibody; Anti-ECE3 Antibody; anti-KEL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human KEL
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-KEL antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human KEL
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human KEL, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-KEL rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:83 kDaObserved band size:85 kDa(We are unsure as to the identity of these extra bands.))

Western Blot (WB) (Anti-KEL rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:83 kDaObserved band size:85 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-KEL antibody
KEL (Kell Metallo-Endopeptidase (Kell Blood Group)) is a Protein Coding gene. This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. It presents two major alleles, KEL1(K) and KEL2(k), but a variety of mutations give rise to weakened (K(mod) phenotype) or lack (K0 phenotype) of Kell antigen expression. The Kell system, encoded by the KEL gene, is one of the most clinically important blood group systems. The very rare KEL:5 results from silent KEL genes also called KELnull alleles. In a few cases, the rare KEL:1, -2 phenotype may be associated with silent KEL*02 alleles. Diseases associated with KEL include Blood Group--Kell System and Choreoacanthocytosis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,824 Da
NCBI Official Full Name
kell blood group glycoprotein
NCBI Official Synonym Full Names
Kell blood group, metallo-endopeptidase
NCBI Official Symbol
KEL
NCBI Official Synonym Symbols
ECE3; CD238
NCBI Protein Information
kell blood group glycoprotein; kell blood group antigen
UniProt Protein Name
Kell blood group glycoprotein
UniProt Gene Name
KEL
UniProt Entry Name
KELL_HUMAN

NCBI Description

This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen. The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases. [provided by RefSeq, Jul 2008]

Uniprot Description

KEL: Zinc endopeptidase with endothelin-3-converting enzyme activity. Cleaves EDN1, EDN2 and EDN3, with a marked preference for EDN3. Belongs to the peptidase M13 family.

Protein type: Membrane protein, integral; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding; metalloendopeptidase activity; metal ion binding

Biological Process: cellular calcium ion homeostasis; myelination; vasoconstriction; regulation of axon diameter; skeletal muscle fiber development; proteolysis; regulation of cell size

Disease: Blood Group--kell System

Research Articles on KEL

Similar Products

Product Notes

The KEL kel (Catalog #AAA8101573) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Kell/KEL Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Kell/KEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the KEL kel for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kell/KEL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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