Rabbit anti-Human EXOG Polyclonal Antibody | anti-EXOG antibody

Anti-EXOG Antibody, Rabbit Polyclonal

Cat. Num. AAA8101399

• Gene Names: EXOG; ENGL; ENGLA; ENGLB; ENGL-a; ENGL-b; ENDOGL1; ENDOGL2
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: EXOG; Polyclonal Antibody; Anti-EXOG Antibody; Rabbit Polyclonal; EXOG Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-ENDOGL1 Antibody; Anti-ENDOGL2 Antibody; Anti-ENGL Antibody; Anti-ENGL-a Antibody; Anti-ENGL-b Antibody; Anti-ENGLA Antibody; Anti-ENGLB Antibody; anti-EXOG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human EXOG
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-EXOG antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human EXOG
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human EXOG, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-EXOG rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDaObserved band size:50 kDa)

Related Product Information for anti-EXOG antibody

Human EXOG (hEXOG) is a 5'-exonuclease that is crucial for mitochondrial DNA repair; the enzyme belongs to a nonspecific nuclease family that includes the apoptotic endonuclease EndoG. hEXOG depletion selectively increases lesions in mtDNA, but has no effect on chromosomal DNA. Conversely, depletion of nucleases that are critical for nuclear BER, flap endonuclease 1 (FEN1) and DNA has no or limited effect on mtDNA. Ectopic expression of hEXOG increases resistance to oxidative stress of proliferating myoblasts, and depletion of EXOG increases oxidative consumption rate in primary neonatal rat ventricular cardiomyocytes. Human EXOG shares sequence homology with members of betabetaalpha-Me nuclease family18, which contains nonspecific endonucleases. One member, EndoG, is the paralog of hEXOG19. The nonspecific activity of EndoG is perfectly suited for activities during apoptosis when it digests single-stranded DNA and double-stranded DNA (dsDNA), and RNA. However, nonspecific activity is incompatible with a precise exonuclease activity necessary to process DNA 5-end in base excision repair (BER). In addition to endonuclease activity, hEXOG also possesses 5-exonuclease activity; relative to the endonucleases, hEXOG harbours a C-terminal extension that may contribute to the new function.

References

Wu CC, et al. (2019) A unique exonuclease exog cleaves between rna and DNA in mitochondrial DNA replication. Nucleic Acids Res 47 (10): 5405-5419.Tigchelaar W, et al. (2015) Loss of mitochondrial exo/endonuclease exog affects mitochondrial respiration and induces ros-mediated cardiomyocyte hypertrophy. Am J Physiol Cell Physiol 308 (2): C155-163.Szymanski MR, et al. (2017) A domain in human exog converts apoptotic endonuclease to DNA-repair exonuclease. Nat Commun 8 14959.

NCBI and Uniprot Product Information

• NCBI GI #: 224451075
• NCBI GeneID: 9941
• NCBI Accession #: NP_001138936.1
• NCBI GenBank Nucleotide #: NM_001145464.1
• UniProt Accession #: Q9Y2C4; Q3SXM9; Q9Y2C8; A8K242; B4DVG2
• Molecular Weight: 41,085 Da
• NCBI Official Full Name: nuclease EXOG, mitochondrial isoform 2
• NCBI Official Synonym Full Names: endo/exonuclease (5'-3'), endonuclease G-like
• NCBI Official Symbol: EXOG
• NCBI Official Synonym Symbols: ENGL; ENGLA; ENGLB; ENGL-a; ENGL-b; ENDOGL1; ENDOGL2
• NCBI Protein Information: nuclease EXOG, mitochondrial; endo G-like 1; endonuclease G-like 1; endonuclease G-like 2
• UniProt Protein Name: Nuclease EXOG, mitochondrial
• Protein Family: Exoglucanase
• UniProt Gene Name: EXOG
• UniProt Synonym Gene Names: ENDOGL1; ENDOGL2; ENGL; Endo G-like 1
• UniProt Entry Name: EXOG_HUMAN

NCBI Description

This gene encodes an endo/exonuclease with 5'-3' exonuclease activity. The encoded enzyme catalyzes the hydrolysis of ester linkages at the 5' end of a nucleic acid chain. This enzyme is localized to the mitochondria and may play a role in programmed cell death. Alternatively spliced transcript variants have been described. A pseudogene exists on chromosome 18. [provided by RefSeq, Feb 2009]

Uniprot Description

ENDOGL1: Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single stranded DNA and 5'-3' exonuclease activity. Belongs to the DNA/RNA non-specific endonuclease family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Deoxyribonuclease; EC 3.1.30.-; Ribonuclease

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: nucleic acid binding; endonuclease activity; metal ion binding

Biological Process: DNA catabolic process, endonucleolytic