Western Blot (WB)
(Anti-WDR77 rabbit polyclonal antibody at 1:500 dilution Lane A: PC12 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:37 kDa Observed band size:40 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human WDR77 Polyclonal Antibody | anti-WDR77 antibody
Anti-WDR77 Antibody, Rabbit Polyclonal
IHC-P: 1:500-1:2000
ICC/IF: 1:300-1:10,000
Western Blot (WB)
(Anti-WDR77 rabbit polyclonal antibody at 1:500 dilution Lane A: PC12 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:37 kDa Observed band size:40 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-WDR77 rabbit polyclonal antibody at 1:500 dilution Lane A: PC12 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:37 kDa Observed band size:40 kDa (We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC)
(Immunochemical staining of human WDR77 in human prostate with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human WDR77 in human prostate with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human WDR77 in human testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human WDR77 in human testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunofluorescence (IF)
(Immunofluorescence staining of WDR77 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human WDR77 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of WDR77 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human WDR77 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
Uniprot Description
WDR77: a WD40 repeat protein. Component of the methylosome, a 20S complex containing at least ICLN, SKB1 and WDR77. Interacts with SKB1 and with SM proteins. The methylosome may regulate an early step in the assembly of U snRNPs, possibly the transfer of Sm proteins to the SMN-complex. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.
Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 1p13.2
Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity
Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; spliceosomal snRNP biogenesis; positive regulation of cell proliferation; gene expression
Research Articles on WDR77
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Product Notes
The WDR77 wdr77 (Catalog #AAA8101274) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-WDR77 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WDR77 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC-P: 1:500-1:2000 ICC/IF: 1:300-1:10,000. Researchers should empirically determine the suitability of the WDR77 wdr77 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WDR77, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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