Rabbit MEIS2 Polyclonal Antibody | anti-MEIS2 antibody

Anti-MEIS2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100837

• Gene Names: MEIS2; MRG1; HsT18361
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: MEIS2; Polyclonal Antibody; Anti-MEIS2 Antibody; Rabbit Polyclonal; MEIS2 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-HsT18361 Antibody; Anti-MRG1 Antibody; anti-MEIS2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MEIS2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MEIS2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1000-1:5000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human MEIS2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human MEIS2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MEIS2 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDaObserved band size:55 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of MEIS2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- MEIS2 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to cytoplasm and nucleus.)

Related Product Information for anti-MEIS2 antibody

MEIS2 is a homeodomain-containing transcription factor of the TALE superfamily that has been proven important for development. MEIS2 has an important role in development and organogenesis, and is implicated in the pathogenesis of human cancer. MEIS2 has been associated with cleft palate and cardiac septal defects as well as varying degrees of intellectual disability.MEIS2 aberrations are considered to be the cause of intellectual disability, cleft palate and cardiac septal defect, as MEIS2 copy number variation is often observed with these phenotypes.

NCBI and Uniprot Product Information

• NCBI GI #: 112180329
• NCBI GeneID: 4212
• UniProt Accession #: O14770; Q96DI2; Q96KI4; Q96KI5; Q9NRS1; Q9NRS2; Q9NRS3; A6NJI5; A8MWD5; B3KP98; B3KPQ6
• Molecular Weight: 51,790 Da
• NCBI Official Full Name: Meis homeobox 2
• NCBI Official Synonym Full Names: Meis homeobox 2
• NCBI Official Symbol: MEIS2
• NCBI Official Synonym Symbols: MRG1; HsT18361
• NCBI Protein Information: homeobox protein Meis2; Meis homolog 2; Meis1-related gene 1; meis1-related protein 1; TALE homeobox protein Meis2; Meis1, myeloid ecotropic viral integration site 1 homolog 2
• UniProt Protein Name: Homeobox protein Meis2
• Protein Family: Homeobox protein
• UniProt Gene Name: MEIS2
• UniProt Synonym Gene Names: MRG1
• UniProt Entry Name: MEIS2_HUMAN

NCBI Description

This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MEIS2: a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription cofactor activity; transcription factor activity; transcription factor binding; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of myeloid cell differentiation; eye development; response to mechanical stimulus; pancreas development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter