Rabbit anti-Human Claudin-3 Polyclonal Antibody | anti-CLDN3 antibody

Anti-Claudin-3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100815

• Gene Names: CLDN3; RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Claudin-3; Polyclonal Antibody; Anti-Claudin-3 Antibody; Rabbit Polyclonal; Claudin 3 Antibody; Rabbit PAb; Antigen Affinity Purified; claudin 3; Anti-C7orf1 Antibody; Anti-CPE-R2 Antibody; Anti-CPETR2 Antibody; Anti-HRVP1 Antibody; Anti-RVP1 Antibody; anti-CLDN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Claudin 3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CLDN3 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:200-1:500
• Immunogen: A synthetic peptide corresponding to the center region of the Human Claudin 3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Claudin 3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CLDN3 in human prostate with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CLDN3 in human small intestine with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-CLDN3 antibody

Claudins (CLDNs) are transmembrane proteins, as normal constituents of the architecture of tight junctions. Recent studies support their involvement in carcinogenesis, as changes in CLDNs structure result in alterations in tight junctions' structure and function, facilitating malignant transformation. We aimed CLDN3 investigation in both breast and ovarian carcinoma, targeting the identification of its expression differences. Hepatocellular carcinoma (HCC) is one of the most common fatal malignancies but the molecular genetic basis of this disease remains unclear. By using genome-wide methylation profiling analysis, we identified CLDN3 as an epigenetically regulated gene in cancer. CLDN3 is an epigenetically silenced metastasis suppressor gene in HCC. A better understanding of the molecular mechanism of CLDN3 in inhibiting liver cancer cell metastasis may lead to a more effective management of HCC patients with the inactivation of CLDN3. CLDN3 expression in ovarian cancer cells, as well as a mechanism for the silencing of this promoter in tumors lacking expression of claudin-3.

NCBI and Uniprot Product Information

• NCBI GI #: 4502875
• NCBI GeneID: 1365
• NCBI Accession #: NP_001297.1
• NCBI GenBank Nucleotide #: NM_001306.3
• UniProt Accession #: O15551
• Molecular Weight: 23,319 Da
• NCBI Official Full Name: claudin-3
• NCBI Official Synonym Full Names: claudin 3
• NCBI Official Symbol: CLDN3
• NCBI Official Synonym Symbols: RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
• NCBI Protein Information: claudin-3; CPE-R 2; CPE-receptor 2; ventral prostate.1-like protein; ventral prostate.1 protein homolog; Clostridium perfringens enterotoxin receptor 2
• UniProt Protein Name: Claudin-3
• Protein Family: Claudin
• UniProt Gene Name: CLDN3
• UniProt Synonym Gene Names: C7orf1; CPETR2; CPE-R 2; CPE-receptor 2; hRVP1
• UniProt Entry Name: CLD3_HUMAN

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]

Uniprot Description

Claudin-3: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Belongs to the claudin family.

Protein type: Cytoskeletal; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: tight junction; integral to plasma membrane; integral to membrane; lateral plasma membrane

Molecular Function: identical protein binding; transmembrane receptor activity; structural molecule activity

Biological Process: response to hypoxia; signal transduction; calcium-independent cell-cell adhesion