Immunofluorescence (IF)
(Immunofluorescence staining of EGR1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human EGR1 polyclonal antibody (1:1000)(Figure A),incubated with rabbit anti-human EGR1 polyclonal antibody and antigen (Figure B) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)
Rabbit anti-Human EGR1 Polyclonal Antibody | anti-EGR1 antibody
Anti-EGR1 Antibody, Rabbit Polyclonal
Immunofluorescence (IF)
(Immunofluorescence staining of EGR1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human EGR1 polyclonal antibody (1:1000)(Figure A),incubated with rabbit anti-human EGR1 polyclonal antibody and antigen (Figure B) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of EGR1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human EGR1 polyclonal antibody (1:1000)(Figure A),incubated with rabbit anti-human EGR1 polyclonal antibody and antigen (Figure B) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq, Jul 2008]
Uniprot Description
EGR1: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. By growth factors. Belongs to the EGR C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding; transcription factor binding; transcription factor activity
Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; regulation of long-term neuronal synaptic plasticity; response to nutrient levels; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; response to amphetamine; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; BMP signaling pathway; learning and/or memory; response to ethanol; cellular response to insulin stimulus; positive regulation of neuron apoptosis; response to glucose stimulus; oligodendrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; response to electrical stimulus; regulation of protein sumoylation; negative regulation of apoptosis; T cell differentiation
Research Articles on EGR1
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Product Notes
The EGR1 egr1 (Catalog #AAA8100782) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EGR1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EGR1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:300-1:10,000. Researchers should empirically determine the suitability of the EGR1 egr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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