Rabbit anti-Human KAP1/TRIM28 Polyclonal Antibody | anti-TRIM28 antibody

Anti-KAP1/TRIM28 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100478

• Gene Names: TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: KAP1/TRIM28; Polyclonal Antibody; Anti-KAP1/TRIM28 Antibody; Rabbit Polyclonal; TRIM28 Antibody; Rabbit PAb; Antigen Affinity Purified; tripartite motif containing 28; Anti-KAP1 Antibody; Anti-PPP1R157 Antibody; Anti-RNF96 Antibody; Anti-TF1B Antibody; Anti-TIF1B Antibody; anti-TRIM28 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TRIM28
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TRIM28 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:2500-1:10,000; IP: 0.5-2uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the human TRIM28
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with A synthetic peptide corresponding to the C-terminus of the human TRIM28, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human TRIM28 in human spleen with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human TRIM28 in human liver with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human TRIM28 in human breast with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-TRIM28 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:89 kDaObserved band size:115 kDa(We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(TRIM28 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate2 uL anti-TRIM28 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TRIM28 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 89 kDaObserved band size: 115 kDa)

Western Blot (WB)

(Anti-TRIM28 rabbit polyclonal antibody at 1:500 dilution Lane A: TRIM28 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:89 kDa Observed band size:115 kDa (We are unsure as to the identity of these extra bands.))

Related Product Information for anti-TRIM28 antibody

Tripartite motif containing 28 (TRIM28) as a transcriptional co-repressor has been reported playing a role in regulating DNA damage response (DDR), cell differentiation, immune response, and tumorigenesis. The germline loss of function mutations in TRIM28 predispose children to Wilms tumour. TRIM28 through its translational suppression of the family of KRAB domain transcription factors in tumor stromal compartments. TRIM28 is a tumor promoter in B-NHL and may be a novel target for drug resistance to Bortezomib. TRIM28 promoted cervical cancer cell growth by activating the mammalian target of rapamycin (mTOR) signaling pathway. that TRIM28 plays a pivotal role in cervical cancer cell proliferation and might serve as a potential therapeutic target.

NCBI and Uniprot Product Information

• NCBI GI #: 3183179
• NCBI GeneID: 10155
• UniProt Accession #: Q13263; O00677; Q7Z632; Q93040; Q96IM1
• Molecular Weight: 835
• NCBI Official Full Name: Transcription intermediary factor 1-beta
• NCBI Official Synonym Full Names: tripartite motif containing 28
• NCBI Official Symbol: TRIM28
• NCBI Official Synonym Symbols: KAP1; TF1B; RNF96; TIF1B; PPP1R157
• NCBI Protein Information: transcription intermediary factor 1-beta; KAP-1; KRIP-1; TIF1-beta; RING finger protein 96; KRAB-associated protein 1; nuclear corepressor KAP-1; KRAB-interacting protein 1; E3 SUMO-protein ligase TRIM28; tripartite motif-containing 28; tripartite motif-c
• UniProt Protein Name: Transcription intermediary factor 1-beta
• Protein Family: Transcription intermediary factor
• UniProt Gene Name: TRIM28
• UniProt Synonym Gene Names: KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1
• UniProt Entry Name: TIF1B_HUMAN

NCBI Description

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Uniprot Description

TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Protein type: EC 6.3.2.-; Ligase; Protein kinase, atypical; Ubiquitin conjugating system; Kinase, protein; Ubiquitin ligase; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; transcription factor activity; transcription corepressor activity; ligase activity; protein kinase activity

Biological Process: transcription initiation from RNA polymerase II promoter; convergent extension involved in axis elongation; positive regulation of DNA repair; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein oligomerization; protein sumoylation; positive regulation of transcription factor import into nucleus; innate immune response; epithelial to mesenchymal transition; gene expression; negative regulation of transcription, DNA-dependent; DNA methylation during embryonic development; embryo implantation