Rabbit anti-Human Nucleophosmin Polyclonal Antibody | anti-NPM1 antibody

Anti-Nucleophosmin Antibody, Rabbit Polyclonal

Cat. Num. AAA8100472

• Gene Names: NPM1; B23; NPM
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: Nucleophosmin; Polyclonal Antibody; Anti-Nucleophosmin Antibody; Rabbit Polyclonal; NPM1/Nucleophosmin Antibody; Rabbit PAb; Antigen Affinity Purified; nucleophosmin (nucleolar phosphoprotein B23; numatrin); Anti-B23 Antibody; Anti-NPM Antibody; anti-NPM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NPM1/Nucleophosmin
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NPM1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Human NPM1/Nucleophosmin protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human NPM1/Nucleophosmin (rh NPM1/Nucleophosmin). NPM1/Nucleophosmin specific IgG was purified by Human NPM1/Nucleophosmin affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NPM1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDaObserved band size:33 kDa)

Related Product Information for anti-NPM1 antibody

Nucleophosmin 1 (NPM1), also known as nucleolar phosphoprotein B23 or numatrin, is a member of the nucleoplasmin family. Nucleophosmin (NPM) is a nucleolar phosphoprotein that plays multiple roles in ribosome assembly and transport, cytoplasmic-nuclear trafficking, centrosome duplication, and regulation of p53. The NPM1 gene is frequently involved in chromosomal translocation, mutation, and deletion. Mutations of the NPM1 gene leading to the expression of a cytoplasmic mutant protein, NPMc+, are the most frequent genetic abnormalities found in acute myeloid leukemias. Acute myeloid leukemia (AML) with mutated NPM1 have distinct characteristics, including a significant association with a normal karyotype, the involvement of different hematopoietic lineages, a specific gene-expression profile, and clinically, a better response to induction therapy, and a favorable prognosis. Also, NPM1 is a crucial gene to consider in the context of the genetics and biology of cancer. NPM1 is frequently overexpressed, mutated, rearranged, and deleted in human cancer. Traditionally regarded as a tumor marker and a putative proto-oncogene, it has now also been attributed with tumor-suppressor functions.

References

Chen W, et al. (2006) Nucleophosmin gene mutations in acute myeloid leukemia. Arch Pathol Lab Med. 130(11): 1687-92.Naoe T, et al. (2006) Nucleophosmin: a versatile molecule associated with hematological malignancies. Cancer Sci. 97(10): 963-9.Grisendi S, et al. (2006) Nucleophosmin and cancer. Nat Rev Cancer. 6(7): 493-505.Falini B, et al. (2007) Acute myeloid leukemia carrying cytoplasmic/mutated nucleophosmin (NPMc+ AML): biologic and clinical features. Blood. 109(3): 874-85.Meani N, et al. (2009) Role of nucleophosmin in acute myeloid leukemia. Expert Rev Anticancer Ther. 9(9): 1283-94.

NCBI and Uniprot Product Information

• NCBI GI #: 83641870
• NCBI GeneID: 4869
• NCBI Accession #: NP_001032827.1
• NCBI GenBank Nucleotide #: NM_001037738.2
• UniProt Accession #: P06748; P08693; Q12826; Q13440; Q13441; Q14115; Q5EU94; Q5EU95; Q5EU96; A8K3N7; B5BU00; D3DQL6
• Molecular Weight: 32,575 Da
• NCBI Official Full Name: nucleophosmin isoform 3
• NCBI Official Synonym Full Names: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• NCBI Official Symbol: NPM1
• NCBI Official Synonym Symbols: B23; NPM
• NCBI Protein Information: nucleophosmin; nucleolar protein NO38; nucleophosmin/nucleoplasmin family, member 1
• UniProt Protein Name: Nucleophosmin
• Protein Family: Nucleophosmin
• UniProt Gene Name: NPM1
• UniProt Synonym Gene Names: NPM; NPM
• UniProt Entry Name: NPM_HUMAN

NCBI Description

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

Uniprot Description

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; Oncoprotein; Nucleolus; Translation

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; cytosol; ribonucleoprotein complex; nucleus

Molecular Function: protein homodimerization activity; protein kinase inhibitor activity; histone binding; RNA binding; transcription coactivator activity; unfolded protein binding; protein kinase binding; ribosomal large subunit binding; NF-kappaB binding; protein binding; protein heterodimerization activity; Tat protein binding; ribosomal small subunit binding

Biological Process: regulation of centriole replication; viral reproduction; positive regulation of translation; centrosome cycle; cell aging; nucleocytoplasmic transport; DNA repair; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; intracellular protein transport; negative regulation of cell proliferation; nucleosome assembly; regulation of endodeoxyribonuclease activity; protein localization; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis