Mouse DDX4 Monoclonal Antibody | anti-DDX4 antibody

Anti-DDX4 Antibody, Mouse Monoclonal

Cat. Num. AAA8100180

• Gene Names: DDX4; VASA
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: DDX4; Monoclonal Antibody; Anti-DDX4 Antibody; Mouse Monoclonal; DDX4 Antibody; Mouse MAb; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; Anti-VASA Antibody; anti-DDX4 antibody

• Host: Mouse
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 03
• Specificity: Human DDX4
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-DDX4 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:50-1:200; ICC/IF: 1:20-1:100
• Immunogen: A synthetic peptide corresponding to the C-terminus of the human DDX4
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human DDX4. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human DDX4 in human testis with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of DDX4 in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.5% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti- DDX4 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)

Related Product Information for anti-DDX4 antibody

DDX4 (DEAD box polypeptide 4), characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), is an RNA helicase which is implicated in various cellular processes involving the alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DDX4 is known to be a germ cell-specific protein and is used as a sorting marker of germline stem cells for the production of oocytes. The active vitamin D treatment prohibited the proliferation and invasion of ovarian cancer cells, and the expression level of a germ cell specific marker DEAD (Asp-Glu-Ala-Asp)-box helicase 4 (DDX4), which is overexpressed in ovarian cancer, was downregulated by active vitamin D treatment. Knockdown of DDX4 by siRNA could also suppress the invasive ability of ovarian cancer cells. Therefore, DDX4 may be considered as a marker of ovarian cancer, and vitamin D may be a candidate drug for ovarian cancer therapy. That DDX4 is essential for the regulation of germ cell proliferation and differentiation across all three extant mammalian groups-eutherians, marsupials, and monotremes.

NCBI and Uniprot Product Information

• NCBI GI #: 216548263
• NCBI GeneID: 54514
• NCBI Accession #: NP_001136021.1
• NCBI GenBank Nucleotide #: NM_001142549.1
• UniProt Accession #: Q9NQI0; Q5M7Z3; Q86VX0; Q9NT92; Q9NYB1; A8K8Q2; B3KSF4; D6RDK4; E9PCD8
• Molecular Weight: 724
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX4 isoform 2
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• NCBI Official Symbol: DDX4
• NCBI Official Synonym Symbols: VASA
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX4; vasa homolog; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX4
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX4
• UniProt Synonym Gene Names: VASA
• UniProt Entry Name: DDX4_HUMAN