Mouse anti-Human beta-Actin Monoclonal Antibody | anti-ACTB antibody

beta-Actin Loading Control Antibody, Mouse MAb

Cat. Num. AAA8100153

• Gene Names: ACTB; BRWS1; PS1TP5BP1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A
• Synonyms: beta-Actin; Monoclonal Antibody; beta-Actin Loading Control Antibody; Mouse MAb; actin; beta; Anti-BRWS1 Antibody; Anti-PS1TP5BP1 Antibody; anti-ACTB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: 10
• Specificity: Human beta-Actin
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ACTB antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:2500-1:20,0000; IP: 1-2uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human beta Actin/ACTB
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the N-terminus of the Human beta Actin/ACTB. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: RAW264.7 Whole Cell Lysate Lane D: Mouse slpeen tissue lysate Lane E: Mouse lung tissue lysate Lane F: Mouse brain tissue lysate Lane G: C6 whole cell lysate Lane H: PC-12 whole cell lysate Lane I: Rat liver tissue lysate Lane J: Rat lung tissue lysate Lane K: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary HRP Conjugated Goat anti-Mouse IgG (H+L),at 1:10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:44 kDa)

Western Blot (WB)

(Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:44 kDa)

Immunoprecipitation (IP)

(ACTB was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 0.5 uL anti-ACTB mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-ACTB mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size: 42 kDa)

Testing Data

(Anti-beta-Actin mouse monoclonal antibody at 1:10000, 1:50000, 1:100000, 1:200000 dilution Lysates/proteins per lane HepG2 (30 ug), Hela (5 ug), Raw246.7 (30 ug) Secondary Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size: 44 kDa)

Related Product Information for anti-ACTB antibody

ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood.

NCBI and Uniprot Product Information

• NCBI GI #: 4501885
• NCBI GeneID: 60
• NCBI Accession #: NP_001092.1
• NCBI GenBank Nucleotide #: NM_001101.3
• UniProt Accession #: P60709; P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
• Molecular Weight: 375
• NCBI Official Full Name: actin, cytoplasmic 1
• NCBI Official Synonym Full Names: actin, beta
• NCBI Official Symbol: ACTB
• NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
• NCBI Protein Information: actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
• UniProt Protein Name: Actin, cytoplasmic 1
• Protein Family: Beta-actin-like protein
• UniProt Gene Name: ACTB
• UniProt Entry Name: ACTB_HUMAN