Rabbit HO-2 Polyclonal Antibody | anti-Hmox2 antibody

HO-2 Antibody: ATTO 488

Cat. Num. AAA806187

• Gene Names: Hmox2; Ho-2
• Reactivity: Rat, Mouse, Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Synonyms: HO-2; Polyclonal Antibody; HO-2 Antibody: ATTO 488; Heme oxygenase 2 antibody; Heme oxygenase (decycling) 2 antibody; Heme oxygenase (decyclizing) 2 antibody; HMOX 2 antibody; HMOX2 antibody; HMOX2_HUMAN antibody; HO 2 antibody; HO2 antibody; anti-Hmox2 antibody

• Host: Rabbit
• Reactivity: Rat, Mouse, Human
• Clonality: Polyclonal
• Form/Format: Protein A Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 315

• Applicable Applications for anti-Hmox2 antibody: Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: 1:1000 (IHC), 1:500 (WB), 1:100 (IP)
• Conjugate: ATTO 488
• Storage Buffer: PBS pH 7.4, 0.09% azide, 50% glycerol
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-HO-2 Polyclonal Antibody . Tissue: Spinal cord. Species: Rat. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:1000.)

Western Blot (WB)

(Western blot analysis of Rat Brain cell lysates showing detection of HO-2 protein using Rabbit Anti-HO-2 Polyclonal Antibody . Lane 1: Rat Brain lysate. Lane 2: Purified HO-2. Load: 10 ug. Primary Antibody: Rabbit Anti-HO-2 Polyclonal Antibody at 1:1000.)

Related Product Information for anti-Hmox2 antibody

Background Info: Detects a ~36kDa protein corresponding to the molecular weight of native rat HO-2

Scientific Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions.

Product Categories/Family for anti-Hmox2 antibody

Oxidative Stress

References

1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411-419.

NCBI and Uniprot Product Information

• NCBI GI #: 13242295
• NCBI GeneID: 79239
• NCBI Accession #: NP_077363.1
• NCBI GenBank Nucleotide #: NM_024387.2
• UniProt Accession #: P23711
• Molecular Weight: 35,762 Da
• NCBI Official Full Name: heme oxygenase 2
• NCBI Official Synonym Full Names: heme oxygenase (decycling) 2
• NCBI Official Symbol: Hmox2
• NCBI Official Synonym Symbols: Ho-2
• NCBI Protein Information: heme oxygenase 2; heme oxygenase-2 non-reducing isoform
• UniProt Protein Name: Heme oxygenase 2
• UniProt Gene Name: Hmox2
• UniProt Synonym Gene Names: HO-2
• UniProt Entry Name: HMOX2_RAT

NCBI Description

catalyzes the conversion of heme to biliverdin; involved in cellular response to oxidative stress [RGD, Feb 2006]

Uniprot Description

HMOX2: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Belongs to the heme oxygenase family.

Protein type: Endoplasmic reticulum; Oxidoreductase; EC 1.14.99.3; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Cellular Component: membrane; endoplasmic reticulum; plasma membrane

Molecular Function: protein binding; metal ion binding; heme oxygenase (decyclizing) activity

Biological Process: response to hypoxia; heme oxidation; response to oxidative stress