Rabbit AHA2 Polyclonal Antibody | anti-AHA2 antibody

AHA2 Antibody

Cat. Num. AAA8002029

• Gene Names: AHSA2P; AHA1; Hch1; AHSA2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A Purified
• Synonyms: AHA2; Polyclonal Antibody; AHA2 Antibody; Rabbit Anti-Human AHA2 Polyclonal; ATPase hydrogen-exporting ATPase 2 Antibody; anti-AHA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Cross reacts with Aha1.
• Purity/Purification: Protein A Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-AHA2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000; ICC/IF: 1:80
• Immunogen: Aha2
• Immunogen Species: Human
• Conjugate: Unconjugated
• Certificate of Analysis: 1 ug/ml of SPC-196 was sufficient for detection of Aha2 in 20 ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA2 Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA2 Polyclonal Antibody at 1:80 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA2 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AHA2 Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA2 Polyclonal Antibody at 1:80 for 12 hours at 4 degree C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-AHA2 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Related Product Information for anti-AHA2 antibody

The Arabidopsis protein RIN4 is a well known regulator of plant immunity. The plasma membrane H+-ATPases Aha1 and Aha2 are one class of RIN4-associated proteins (1-5). Aha1 and Aha2 play a crucial role in resisting pathogen invasion-plants use RIN4 to regulate H+-ATPase activity during immune responses, thereby controlling stomatal apertures during pathogen attack (1). Wild type AHA2 has been found to be localized to the plasma membrane, and has also been found in the ER (4).

Product Categories/Family for anti-AHA2 antibody

Cancer; Heat Shock; Cell Signaling; Protein Trafficking; Chaperone Proteins

References

1. Liu J., Elmore J.M., and Coaker G. (2009) Plant Signal Behav. 4(12): 1107-1110. 2. Harper J.F., Surowy T.K. and Sussman M.R. (1989) Proc Natl Acad. Sci USA. 86: 1234-1238. 3. Harper J. F., et al. (1990) J Biol Chem. 265: 13601-13608. 4. Harper J.F., Manney L., and Sussman M.R. (1994) Mol Gen Genet. 243: 572-587. 5. Regenberg B., Villalba J.M., Lanfermeijer F.C., and Palmgren M.G. (1995) Plant Cell. 7: 1655-1666.

NCBI and Uniprot Product Information

• NCBI GI #: 22748839
• NCBI GeneID: 130872
• NCBI Accession #: NP_689605.1
• NCBI GenBank Nucleotide #: NM_152392.4
• UniProt Accession #: Q719I0
• NCBI Official Synonym Full Names: activator of HSP90 ATPase homolog 2, pseudogene
• NCBI Official Symbol: AHSA2P
• NCBI Official Synonym Symbols: AHA1; Hch1; AHSA2
• UniProt Protein Name: Activator of 90 kDa heat shock protein ATPase homolog 2
• Protein Family: ATPase
• UniProt Gene Name: AHSA2
• UniProt Entry Name: AHSA2_HUMAN

Uniprot Description

AHSA2: Co-chaperone that stimulates HSP90 ATPase activity. Belongs to the AHA1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Activator

Chromosomal Location of Human Ortholog: 2p15

Molecular Function: ATPase activator activity; chaperone binding

Biological Process: positive regulation of ATPase activity