Human Toll Interleukin 1 Receptor Domain Containing Adaptor Protein ELISA Kit | TIRAP elisa kit

Human Toll Interleukin 1 Receptor Domain Containing Adaptor Protein ELISA Kit

Cat. Num. AAA78939

• Gene Names: TICAM1; TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1
• Reactivity: Human
• Synonyms: Toll Interleukin 1 Receptor Domain Containing Adaptor Protein; Human Toll Interleukin 1 Receptor Domain Containing Adaptor Protein ELISA Kit; TIRAP elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human TIRAP and analogues was observed.
• Sequence Length: 712

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml - 20 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated TIRAP concentrations in Human serum, plasma and other body fluids. Using Purified Human TIRAP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TIRAP and TIRAP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TIRAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for TIRAP elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated TIRAP concentrations in Human serum, plasma and other body fluids. Using Purified Human TIRAP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TIRAP and TIRAP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TIRAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 74727957
• NCBI GeneID: 148022
• UniProt Accession #: Q8IUC6; O75532; Q86XP8; Q96GA0; B3Y691
• Molecular Weight: 76,422 Da
• NCBI Official Full Name: TIR domain-containing adapter molecule 1
• NCBI Official Synonym Full Names: toll-like receptor adaptor molecule 1
• NCBI Official Symbol: TICAM1
• NCBI Official Synonym Symbols: TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1
• NCBI Protein Information: TIR domain-containing adapter molecule 1; putative NF-kappa-B-activating protein 502H; TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter protein inducing IFN-beta; proline-rich, vinculin and TIR domain-containing protein B; toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta
• UniProt Protein Name: TIR domain-containing adapter molecule 1
• Protein Family: Toll/interleukin-1 receptor domain-containing adapter protein
• UniProt Gene Name: TICAM1
• UniProt Synonym Gene Names: PRVTIRB; TRIF; TICAM-1; MyD88-3
• UniProt Entry Name: TCAM1_HUMAN

NCBI Description

This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response. [provided by RefSeq, Jan 2012]

Uniprot Description

TICAM1: an adaptor protein involved in innate immunity against invading pathogens. Associates with TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TICAM1 recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Interacts with the TIR domain of TLR3. Interacts with AZI2, TBK1, IRF3 and IRF7. Interacts with TICAM2 in TLR4 recruitment. Interaction with PIAS4 inhibits the TICAM1-induced NF-kappa-B, IRF and IFNB1 activation. Interacts with IKBKB and IKBKE. Interaction with SARM1 blocks TICAM1-dependent transcription factor activation. Interacts with TRAF3. Interacts with TRAFD1.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: endosome membrane; cytosol

Molecular Function: signal transducer activity; protein binding; protein kinase binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of nitric oxide biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein binding; MyD88-independent toll-like receptor signaling pathway; regulation of protein homodimerization activity; positive regulation of interleukin-6 production; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; positive regulation of natural killer cell activation; activation of NF-kappaB transcription factor; response to exogenous dsRNA; positive regulation of chemokine biosynthetic process; positive regulation of protein ubiquitination; macrophage activation during immune response; positive regulation of interferon-beta biosynthetic process; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; positive regulation of B cell proliferation; innate immune response; inflammatory response; toll-like receptor 4 signaling pathway; defense response to virus

Disease: Herpes Simplex Encephalitis, Susceptibility To, 4