Rabbit CISD2 Polyclonal Antibody | anti-CISD2 antibody

CISD2 Rabbit Polyclonal

Cat. Num. AAA767950

• Gene Names: CISD2; ERIS; WFS2; ZCD2; NAF-1; Miner1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: CISD2; Polyclonal Antibody; CISD2 Rabbit Polyclonal; anti-CISD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 135

• Applicable Applications for anti-CISD2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Immunogen: CDGSH iron sulfur domain 2
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS767950 (CISD2 antibody) at dilution of 1:100)

Testing Data

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Western Blot (WB)

(mouse kidney tissue were subjected to SDS PAGE followed by western blot with MBS767950 (CISD2 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 56605994
• NCBI GeneID: 493856
• NCBI Accession #: NP_001008389.1
• NCBI GenBank Nucleotide #: NM_001008388.4
• Molecular Weight: 15,278 Da
• NCBI Official Full Name: CDGSH iron-sulfur domain-containing protein 2
• NCBI Official Synonym Full Names: CDGSH iron sulfur domain 2
• NCBI Official Symbol: CISD2
• NCBI Official Synonym Symbols: ERIS; WFS2; ZCD2; NAF-1; Miner1
• NCBI Protein Information: CDGSH iron-sulfur domain-containing protein 2
• UniProt Protein Name: CDGSH iron-sulfur domain-containing protein 2
• Protein Family: CDGSH iron-sulfur domain-containing protein
• UniProt Gene Name: CISD2
• UniProt Synonym Gene Names: CDGSH2; ERIS; ZCD2; Miner1; NAF-1
• UniProt Entry Name: CISD2_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2. [provided by RefSeq, Mar 2011]

Uniprot Description

CISD2: a single-pass membrane protein of the endoplasmic reticulum that regulates autophagy. Has also been reported to localize to the mitochondrion outer membrane. Antagonizes ATG6-mediated cellular autophagy. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca2+ stores during autophagy. Contributes to BIK-initiated autophagy, while it is not involved in BIK-dependent activation of caspases. Involved in life span control, probably via its function as regulator of autophagy. Directly interacts with BCL2; the interaction is disrupted by BIK interaction with BCL2. It has been reported to mainly localizes to the mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome 2 (WFS2), is a rare autosomal recessive disorder characterized by optic atrophy and diabetes mellitus. Other symptoms include the presence of profound upper gastrointestinal ulceration, significant bleeding tendency, defective platelet aggregation with collagen and various neurological symptoms. Initially thought to be a zinc-finger protein, it was later shown to bind 1 2Fe-2S cluster instead.

Protein type: Membrane protein, integral; Autophagy; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; membrane; mitochondrial outer membrane; protein complex

Molecular Function: 2 iron, 2 sulfur cluster binding; metal ion binding; protein binding; protein homodimerization activity

Biological Process: mitochondrion degradation; multicellular organismal aging; regulation of autophagy

Disease: Wolfram Syndrome 2