Typical Testing Data/Standard Curve (for reference only)
Mouse CYB5A (Cytochrome b5) ELISA Kit | CYB5A elisa kit
Mouse CYB5A (Cytochrome b5)
Gene Names
LOC100328915; CYB5; CYB5A
Reactivity
Mouse
Synonyms
CYB5A (Cytochrome b5); Mouse CYB5A (Cytochrome b5); CYB5A elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection ofCYB5A . No significant cross-reactivity or interference betweenCYB5A and analogues was observed.
Sequence Length
134
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
15.6-1000pg/ml
Sensitivity
9.4pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelCYB5A were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelCYB5A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
4 degree C for 6 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CYB5A elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CYB5A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CYB5A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CYB5A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofCYB5A can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,232 Da
NCBI Official Full Name
cytochrome b5
NCBI Official Symbol
LOC100328915
NCBI Official Synonym Symbols
CYB5; CYB5A
NCBI Protein Information
cytochrome b5
UniProt Protein Name
Cytochrome b5
Protein Family
UniProt Gene Name
CYB5A
UniProt Synonym Gene Names
CYB5
UniProt Entry Name
CYB5_RABIT
Uniprot Description
Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Research Articles on CYB5A
Similar Products
Product Notes
The Mouse CYB5A cyb5a (Catalog #AAA766189) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA766189 ELISA Kit recognizes Mouse CYB5A. It is sometimes possible for the material contained within the vial of "CYB5A (Cytochrome b5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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