Human Cleaved CASP9 (Cleaved Caspase-9) ELISA Kit | CASP9 elisa kit

Human Cleaved CASP9 (Cleaved Caspase-9)

Cat. Num. AAA766156

• Gene Names: CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• Reactivity: Human
• Synonyms: Cleaved CASP9 (Cleaved Caspase-9); Human Cleaved CASP9 (Cleaved Caspase-9); CASP9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cleaved CASP9. No significant cross-reactivity or interference between Cleaved CASP9 and analogues was observed.
• Sequence Length: 154

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: 4 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for CASP9 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 54300706
• NCBI GeneID: 842
• Molecular Weight: 36,564 Da
• NCBI Official Full Name: caspase 9 splice variant
• NCBI Official Synonym Full Names: caspase 9
• NCBI Official Symbol: CASP9
• NCBI Official Synonym Symbols: MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• NCBI Protein Information: caspase-9
• UniProt Protein Name: Caspase-9
• Protein Family: Caspase
• UniProt Gene Name: CASP9
• UniProt Synonym Gene Names: MCH6; CASP-9; APAF-3; ICE-LAP6
• UniProt Entry Name: CASP9_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms.

Protein type: Protease; Apoptosis; EC 3.4.22.62

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: apoptosome; cytosol; mitochondrion; nucleus

Molecular Function: cysteine-type endopeptidase activity; enzyme activator activity; peptidase activity; protein binding; protein kinase binding; SH3 domain binding

Biological Process: aging; apoptosis; caspase activation via cytochrome c; DNA damage response, signal transduction; DNA damage response, signal transduction resulting in induction of apoptosis; platelet formation; positive regulation of neuron apoptosis; response to antibiotic; response to cobalt ion; response to DNA damage stimulus; response to estradiol stimulus; response to lipopolysaccharide