Rat Glycylpeptide N-tetradecanoyltransferase 1 ELISA Kit | Nmt1 elisa kit

Rat Glycylpeptide N-tetradecanoyltransferase 1 ELISA Kit

Cat. Num. AAA766010

• Gene Names: NMT1; NMT
• Reactivity: Rat
• Synonyms: Glycylpeptide N-tetradecanoyltransferase 1; Rat Glycylpeptide N-tetradecanoyltransferase 1 ELISA Kit; Nmt1/Myristoyl-CoA:protein N-myristoyltransferase 1 (NMT 1/Type I N-myristoyltransferase)/Peptide N-myristoyltransferase 1/; Nmt1 elisa kit

• Reactivity: Rat
• Sequence Length: 496

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Nmt1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Nmt1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Nmt1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Nmt1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Nmt1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10835073
• NCBI GeneID: 4836
• NCBI Accession #: NP_066565.1
• NCBI GenBank Nucleotide #: NM_021079.4
• Molecular Weight: 48,141 Da
• NCBI Official Full Name: glycylpeptide N-tetradecanoyltransferase 1
• NCBI Official Synonym Full Names: N-myristoyltransferase 1
• NCBI Official Symbol: NMT1
• NCBI Official Synonym Symbols: NMT
• NCBI Protein Information: glycylpeptide N-tetradecanoyltransferase 1
• UniProt Protein Name: Glycylpeptide N-tetradecanoyltransferase 1
• Protein Family: Glycylpeptide N-tetradecanoyltransferase
• UniProt Gene Name: NMT1
• UniProt Synonym Gene Names: NMT; NMT 1; Type I N-myristoyltransferase
• UniProt Entry Name: NMT1_HUMAN

NCBI Description

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008]

Uniprot Description

NMT1: Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Belongs to the NMT family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.3.1.97; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: actin cytoskeleton; cell junction; cytoplasm; cytosol; extrinsic to membrane; mitochondrion; plasma membrane

Molecular Function: glycylpeptide N-tetradecanoyltransferase activity

Biological Process: apoptosis; in utero embryonic development; N-terminal peptidyl-glycine N-myristoylation; phototransduction, visible light; programmed cell death; regulation of rhodopsin mediated signaling; rhodopsin mediated signaling; viral reproduction