Human Tristetraprolin ELISA Kit | ZFP36 elisa kit

Human Tristetraprolin ELISA Kit

Cat. Num. AAA765863

• Gene Names: ZFP36; TTP; G0S24; GOS24; TIS11; NUP475; zfp-36; RNF162A
• Reactivity: Human
• Synonyms: Tristetraprolin; Human Tristetraprolin ELISA Kit; ZFP36/TTP/G0/G1 switch regulatory protein 24/Growth factor-inducible nuclear protein NUP475/Protein TIS11A/TIS11/Zinc finger protein 36 homolog/Zfp-36/ZFP36/G0S24/; ZFP36 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WT1. No significant cross-reactivity or interference between WT1 and analogues was observed.
• Sequence Length: 332

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.6-1000pg/ml
• Sensitivity: <9.375pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ZFP36 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-WT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-WT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the WT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of WT1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 393539038
• NCBI GeneID: 7538
• NCBI Accession #: NP_003398.2
• NCBI GenBank Nucleotide #: NM_003407.3
• Molecular Weight: 34,003 Da
• NCBI Official Full Name: tristetraprolin
• NCBI Official Synonym Full Names: ZFP36 ring finger protein
• NCBI Official Symbol: ZFP36
• NCBI Official Synonym Symbols: TTP; G0S24; GOS24; TIS11; NUP475; zfp-36; RNF162A
• NCBI Protein Information: tristetraprolin
• UniProt Protein Name: Tristetraprolin
• Protein Family: mRNA decay activator protein
• UniProt Gene Name: ZFP36
• UniProt Synonym Gene Names: G0S24; RNF162A; TIS11A; TTP; TTP; TIS11; Zfp-36
• UniProt Entry Name: TTP_HUMAN

Uniprot Description

TTP: a CCCH zinc finger protein that has anti-inflammatory and arthritis suppressor activity. Regulates inflammatory responses at the post-transcriptional level. TTP deficiency in mice causes a severe inflammatory syndrome that includes autoimmunity, myeloid hyperplasia, and erosive arthritis. Binds to and destabilizes mRNA molecules with AU-rich elements (AREs). Has a high affinity for UUAUUUAUU mRNA sequences. Destabilizes tumor necrosis factor-alpha (TNF??), interleukin 2 (IL2), granulocyte-macrophage colony-stimulating factor (GM-CSF), transcription factor E47, and cyclooxygenase 2 (COX2) mRNAs. TNF?? and GM-CSF mRNAs are stabilized in TTP-deficient mice, causing a severe inflammatory syndrome. Conversely, high levels of TTP reduce inflammatory responses. TTP is a very low abundance protein that is rapidly induced by insulin, glucocorticoids, lipopolysaccharide (LPS), fetal calf serum, zinc, herpes simplex virus 1, green tea, and cinnamon. Once induced, the TTP protein is relatively stable. Has been experimentaly shown to be able to bind zinc. TTP is extensively phosphorylated.

Protein type: RNA-binding; DNA-binding

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm; cytosol; nucleus; stress granule

Molecular Function: AU-rich element binding; C-C chemokine binding; DNA binding; enzyme binding; metal ion binding; mRNA binding; protein binding; protein kinase binding; single-stranded RNA binding

Biological Process: gene expression; miRNA-mediated gene silencing, negative regulation of translation; mRNA catabolic process; mRNA catabolic process, deadenylation-dependent decay; negative regulation of transcription from RNA polymerase II promoter; poly(A) tail shortening; regulation of mRNA stability; regulation of tumor necrosis factor production; response to starvation