Human Interleukin-1 receptor-associated kinase 1 ELISA Kit | IRAK1 elisa kit

Human Interleukin-1 receptor-associated kinase 1 ELISA Kit

Cat. Num. AAA765799

• Gene Names: IRAK1; IRAK; pelle
• Reactivity: Human
• Synonyms: Interleukin-1 receptor-associated kinase 1; Human Interleukin-1 receptor-associated kinase 1 ELISA Kit; IRAK1/Interleukin-1 Receptor-Associated Kinase 1/Il1rak/Pelle/Plpk/IRAK-1/IRAKPelle homolog; IRAK1 elisa kit

• Reactivity: Human
• Sequence Length: 712

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for IRAK1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human IRAK1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human IRAK1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IRAK1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human IRAK1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 68800243
• NCBI GeneID: 3654
• NCBI Accession #: NP_001560.2
• NCBI GenBank Nucleotide #: NM_001569.3
• Molecular Weight: 68,022 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 1 isoform 1
• NCBI Official Synonym Full Names: interleukin 1 receptor associated kinase 1
• NCBI Official Symbol: IRAK1
• NCBI Official Synonym Symbols: IRAK; pelle
• NCBI Protein Information: interleukin-1 receptor-associated kinase 1
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 1
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK1
• UniProt Synonym Gene Names: IRAK; IRAK-1
• UniProt Entry Name: IRAK1_HUMAN

NCBI Description

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; TKL group; IRAK family

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cytoplasm; cytosol; endosome membrane; interleukin-1 receptor complex; lipid particle; nucleus; plasma membrane

Molecular Function: ATP binding; heat shock protein binding; interleukin-1 receptor binding; kinase activity; NF-kappaB-inducing kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity; ubiquitin-protein ligase activity

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; activation of NF-kappaB-inducing kinase; aging; inhibition of NF-kappaB transcription factor; innate immune response; JNK cascade; lipopolysaccharide-mediated signaling pathway; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; nerve growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of MAP kinase activity; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein oligomerization; protein ubiquitination; regulation of cytokine and chemokine mediated signaling pathway; response to lipopolysaccharide; response to peptidoglycan; signal transduction; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transmembrane receptor protein serine/threonine kinase signaling pathway