Rat Cholinergic Receptor, Muscarinic 2 ELISA Kit | CHRM2 elisa kit

Rat Cholinergic Receptor, Muscarinic 2 ELISA Kit

Cat. Num. AAA765680

• Gene Names: CHRM2; HM2
• Reactivity: Rat
• Synonyms: Cholinergic Receptor; Muscarinic 2; Rat Cholinergic Receptor; Muscarinic 2 ELISA Kit; CHRM2/HM2/mAChR M2/7TM receptor/cholinergic receptor; muscarinic 2/muscarinic acetylcholine receptor M2/muscarinic M2 receptor; CHRM2 elisa kit

• Reactivity: Rat
• Sequence Length: 466

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 62.5-4000pmol/ml
• Sensitivity: 37.5 pmol/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CHRM2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat CHRM2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat CHRM2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat CHRM2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat CHRM2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 51094808
• NCBI GeneID: 1129
• Molecular Weight: 51,715 Da
• NCBI Official Full Name: cholinergic receptor, muscarinic 2
• NCBI Official Synonym Full Names: cholinergic receptor, muscarinic 2
• NCBI Official Symbol: CHRM2
• NCBI Official Synonym Symbols: HM2
• NCBI Protein Information: muscarinic acetylcholine receptor M2
• UniProt Protein Name: Muscarinic acetylcholine receptor M2
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: CHRM2
• UniProt Entry Name: ACM2_HUMAN

NCBI Description

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

mAChR M2: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD). MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q31-q35

Cellular Component: asymmetric synapse; cell junction; cell soma; dendrite; integral to plasma membrane; nerve terminal; plasma membrane; postsynaptic membrane; synapse

Molecular Function: drug binding; G-protein coupled acetylcholine receptor activity

Biological Process: acetylcholine receptor signaling, muscarinic pathway; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein signaling, coupled to cyclic nucleotide second messenger; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; muscarinic acetylcholine receptor, phospholipase C activating pathway; nervous system development; regulation of heart contraction; regulation of smooth muscle contraction; response to virus; synaptic transmission, cholinergic