Rat Agrin ELISA Kit | AGRN elisa kit

Rat Agrin ELISA Kit

Cat. Num. AAA765631

• Gene Names: AGRN; HSPG
• Reactivity: Rat
• Synonyms: Agrin; Rat Agrin ELISA Kit; AGRN/Agrin/agrin proteoglycan; AGRN elisa kit

• Reactivity: Rat
• Sequence Length: 1955

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for AGRN elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat AGRN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat AGRN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat AGRN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat AGRN can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 211121
• NCBI GeneID: 396538
• Molecular Weight: 224,132 Da
• NCBI Official Full Name: agrin
• NCBI Official Synonym Full Names: agrin
• NCBI Official Symbol: AGRN
• NCBI Official Synonym Symbols: HSPG
• NCBI Protein Information: agrin
• UniProt Protein Name: Agrin
• Protein Family: Agrin
• UniProt Gene Name: AGRN
• UniProt Synonym Gene Names: AGRIN; C90; C22
• UniProt Entry Name: AGRIN_CHICK

Uniprot Description

Isoform 1: heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. Component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses.Isoform 9: transmembrane agrin (TM-agrin), the predominant form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.Isoform 2, isoform 4 and isoform 7: muscle agrin isoforms, which lack the 8-amino acid insert at the 'B' site, but with the insert at the'A' site have no AChr clustering activity nor MUSK activation but bind heparin. Bind alpha-dystroglycan with lower affinity.Isoform 5: muscle agrin A0B0 lacking inserts at both 'A' and 'B' sites has no heparin-binding nor AChR clustering activity but binds strongly alpha-dystroglycan.Agrin N-terminal 110 kDa subunit: is involved in modulation of growth factor signaling (). Involved also in the regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling.