Human Serine/Threonine-protein Kinase Pim-1 ELISA Kit | PIM-1 elisa kit

Human Serine/Threonine-protein Kinase Pim-1 ELISA Kit

Cat. Num. AAA765402

• Reactivity: Human
• Synonyms: Serine/Threonine-protein Kinase Pim-1; Human Serine/Threonine-protein Kinase Pim-1 ELISA Kit; PIM-1 elisa kit

• Reactivity: Human
• Sequence Length: 313

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.180ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PIM-1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PIM-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PIM-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PIM-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PIM-1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 8393959
• NCBI GeneID: 24649
• NCBI Accession #: NP_058730.1
• NCBI GenBank Nucleotide #: NM_017034.1
• Molecular Weight: 35,631 Da
• NCBI Official Full Name: serine/threonine-protein kinase pim-1
• NCBI Official Synonym Full Names: Pim-1 proto-oncogene, serine/threonine kinase
• NCBI Official Symbol: Pim1
• NCBI Protein Information: serine/threonine-protein kinase pim-1
• UniProt Protein Name: Serine/threonine-protein kinase pim-1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Pim1
• UniProt Synonym Gene Names: Pim-1
• UniProt Entry Name: PIM1_RAT

NCBI Description

serine threonine protein kinase; 2.8 kb transcript is detected in somatic cells and a 2.3 kb transcript using an alternate polyadenylation signal is expressed in testis [RGD, Feb 2006]

Uniprot Description

Pim1: a proto-oncogene serine/threonine kinase involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerced by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl- X(L)/BCL2L1. Phosphorylation of ASK1 an other proapoptotic protein, by PIM1, significantly decreases ASK1 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of p21Cip1, a regulator of cell cycle progression at G1, results in the relocation of p21Cip1 to the cytoplasm and enhanced p21Cip1 protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, p27Kip1, at both transcriptional and post- translational levels. Phosphorylation of p27Kip1,induces 14-3-3- proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Binds to RP9. Isoform 1, but not isoform 2, binds BMX. Isoform 2 interacts with p27Kip1 and FOXO3. Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM1, ubiquitination and proteasomal degradation. Interacts with HSP90, this interaction stabilizes PIM1 protein levels. Ubiquitinated form interacts with HSP70 and promotes its proteosomal degradation. Strongly induced in leukocytes by the JAK/STAT pathway in response to cytokines. Induced by different cellular stresses, heat shock and cytotoxic agents. Expressed primarily in cells of the hematopoietic and germline lineages. 2 isoforms of the human protein are produced by alternative initiation. Both isoforms are expressed in prostate cancer cell lines.

Protein type: EC 2.7.11.1; Oncoprotein; Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; PIM family

Cellular Component: cytoplasm; nucleus; plasma membrane

Molecular Function: ATP binding; manganese ion binding; protein serine/threonine kinase activity; ribosomal small subunit binding; transcription factor binding

Biological Process: apoptosis; cell proliferation; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; hyaluronan metabolic process; negative regulation of apoptosis; negative regulation of transcription factor activity; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of mitotic cell cycle