Human Integrin beta-3 ELISA Kit | ITGB3 elisa kit

Human Integrin beta-3 ELISA Kit

Cat. Num. AAA765397

• Gene Names: ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16
• Reactivity: Human
• Synonyms: Integrin beta-3; Human Integrin beta-3 ELISA Kit; ITGB3/Integrin beta-3/Platelet membrane glycoprotein IIIa/GPIIIa/GP3A/CD61/INGRB3/ITG B3; ITGB3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ITGB3. No significant cross-reactivity or interference between ITGB3 and analogues was observed.
• Sequence Length: 788

• Samples: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78-5000pg/ml
• Sensitivity: < 46.9pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITGB3were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITGB3were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGB3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ITGB3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ITGB3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ITGB3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ITGB3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 47078292
• NCBI GeneID: 3690
• NCBI Accession #: NP_000203.2
• NCBI GenBank Nucleotide #: NM_000212.2
• Molecular Weight: 86,694 Da
• NCBI Official Full Name: integrin beta-3
• NCBI Official Synonym Full Names: integrin subunit beta 3
• NCBI Official Symbol: ITGB3
• NCBI Official Synonym Symbols: GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16
• NCBI Protein Information: integrin beta-3
• UniProt Protein Name: Integrin beta-3
• Protein Family: Integrin
• UniProt Gene Name: ITGB3
• UniProt Synonym Gene Names: GP3A; GPIIIa
• UniProt Entry Name: ITB3_HUMAN

NCBI Description

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cell surface; filopodium membrane; focal adhesion; integral to plasma membrane; integrin complex; melanosome; microvillus membrane; nucleus; plasma membrane; platelet alpha granule membrane; receptor complex

Molecular Function: cell adhesion molecule binding; enzyme binding; extracellular matrix binding; fibronectin binding; identical protein binding; platelet-derived growth factor receptor binding; protease binding; protein binding; protein disulfide isomerase activity; vascular endothelial growth factor receptor 2 binding; viral receptor activity

Biological Process: activation of protein kinase activity; angiogenesis involved in wound healing; axon guidance; blood coagulation; cell adhesion; cell growth; cell migration; cell-matrix adhesion; cell-substrate adhesion; cell-substrate junction assembly; entry of virus into host cell; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; integrin-mediated signaling pathway; leukocyte migration; mesodermal cell differentiation; negative chemotaxis; negative regulation of lipid transport; negative regulation of lipoprotein metabolic process; negative regulation of low-density lipoprotein receptor biosynthetic process; platelet activation; platelet degranulation; positive regulation of endothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; protein folding; regulation of bone resorption; smooth muscle cell migration; tube development; vascular endothelial growth factor receptor signaling pathway; wound healing

Disease: Bleeding Disorder, Platelet-type, 16; Glanzmann Thrombasthenia; Myocardial Infarction, Susceptibility To