Human Apolipoprotein L1 ELISA Kit | APOL1 elisa kit

Human Apolipoprotein L1 ELISA Kit

Cat. Num. AAA765169

• Gene Names: APOL1; APOL; APO-L; FSGS4; APOL-I
• Reactivity: Human
• Synonyms: Apolipoprotein L1; Human Apolipoprotein L1 ELISA Kit; APOL1 (Apolipoprotein L1)/APOL/Apo-L/ApoL-I/Apolipoprotein L/Apolipoprotein L-I; APOL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of APOL1. No significant cross-reactivity or interference between APOL1 and analogues was observed.
• Sequence Length: 398

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.78-50ng/ml
• Sensitivity: <0.469ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level APOL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level APOL1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Related Product Information for APOL1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- APOL1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- APOL1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the APOL1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of APOL1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 13374347
• NCBI GeneID: 8542
• Molecular Weight: 42,158 Da
• NCBI Official Full Name: apolipoprotein L1
• NCBI Official Synonym Full Names: apolipoprotein L1
• NCBI Official Symbol: APOL1
• NCBI Official Synonym Symbols: APOL; APO-L; FSGS4; APOL-I
• NCBI Protein Information: apolipoprotein L1
• UniProt Protein Name: Apolipoprotein L1
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOL1
• UniProt Synonym Gene Names: APOL; Apo-L; ApoL; ApoL-I
• UniProt Entry Name: APOL1_HUMAN

NCBI Description

This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

APOL1: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, chloride; Lipid-binding; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular region; extracellular space; intrinsic to membrane

Molecular Function: chloride channel activity; lipid binding; protein binding

Biological Process: cholesterol metabolic process; cytolysis; innate immune response; killing of cells of another organism; lipid transport; lipoprotein metabolic process; receptor-mediated endocytosis

Disease: Focal Segmental Glomerulosclerosis 4, Susceptibility To