Related Product Information for TFF3 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human TFF3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TFF3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TFF3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human TFF3 can be calculated.
NCBI Description
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
Uniprot Description
TFF3: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: cytoplasm; extracellular region; proteinaceous extracellular matrix
Molecular Function: protein binding
Biological Process: defense response; digestion
Product Notes
The Human TFF3 tff3 (Catalog #
AAA765033) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA765033 ELISA Kit recognizes Human TFF3.
It is sometimes possible for the material contained within the vial of
"Trefoil factor 3, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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