Human ATP-binding cassette sub-family A member 1 ELISA Kit | ABCA1 elisa kit

Human ATP-binding cassette sub-family A member 1 ELISA Kit

Cat. Num. AAA764197

• Gene Names: ABCA1; TGD; ABC1; CERP; ABC-1; HDLDT1
• Reactivity: Human
• Synonyms: ATP-binding cassette sub-family A member 1; Human ATP-binding cassette sub-family A member 1 ELISA Kit; ABCA1 (ATP-binding cassette transporter A1)/ABC1/CERP/HDLDT1/TGD/ABC-1/ATP-binding cassette 1/ATP-binding cassette transporter 1/ATP-binding cassette; sub-family A; member 1/Cholesterol efflux regulatory protein/membrane-bound/TGDATP-binding cassette sub-family A member 1; ABCA1 elisa kit

• Reactivity: Human
• Sequence Length: 2261

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ABCA1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ABCA1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ABCA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ABCA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ABCA1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21536376
• NCBI GeneID: 19
• NCBI Accession #: NP_005493.2
• NCBI GenBank Nucleotide #: NM_005502.3
• Molecular Weight: 254,302 Da
• NCBI Official Full Name: ATP-binding cassette sub-family A member 1
• NCBI Official Synonym Full Names: ATP binding cassette subfamily A member 1
• NCBI Official Symbol: ABCA1
• NCBI Official Synonym Symbols: TGD; ABC1; CERP; ABC-1; HDLDT1
• NCBI Protein Information: ATP-binding cassette sub-family A member 1
• UniProt Protein Name: ATP-binding cassette sub-family A member 1
• Protein Family: ABC transporter A family
• UniProt Gene Name: ABCA1
• UniProt Synonym Gene Names: ABC1; CERP; ABC-1; ATP-binding cassette 1
• UniProt Entry Name: ABCA1_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCA1: an ATP-binding cassette (ABC) transporter that regulates cholesterol homeostasis and insulin secretion in beta-cells, and protects against cardiovascular disease. Inactivation of ABCA1 in beta-cells markedly impairs glucose tolerance and insulin secretion in mice. Rosiglitazone, an activator of the peroxisome proliferator-activated receptor-gamma, upregulates ABCA1 in beta-cells. ABCA1 expression in beta-cells is required for the beneficial effects of rosiglitazone on glucose tolerance. Mediates the transport of cholesterol and phospholipids from cells to HDL apolipoproteins, modulating HDL levels and atherogenesis. Induced by bacterial lipopolysaccharides (LPS) and repressed by ZNF202. Phosphorylation on Ser-2054 regulates phospholipid efflux. Unsaturated fatty acids destabilize ABCA1 by activating a PKC-delta pathway that phosphorylates ABCA1 serines. Defects in ABCA1 are a cause of high density lipoprotein deficiencies.

Protein type: Membrane protein, integral; Vesicle; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: endocytic vesicle; external side of plasma membrane; Golgi apparatus; integral to plasma membrane; lipid raft; perinuclear region of cytoplasm; phagocytic vesicle; plasma membrane

Molecular Function: anion transmembrane transporter activity; apolipoprotein A-I binding; apolipoprotein A-I receptor activity; apolipoprotein binding; ATP binding; ATPase activity, coupled to transmembrane movement of substances; ATPase binding; cholesterol binding; cholesterol transporter activity; phospholipid binding; phospholipid transporter activity; protein binding; receptor binding; small GTPase binding; syntaxin binding

Biological Process: cellular lipid metabolic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; endosome transport; G-protein coupled receptor protein signaling pathway; interleukin-1 beta secretion; intracellular cholesterol transport; lipoprotein metabolic process; lysosome organization and biogenesis; peptide secretion; phagocytosis, engulfment; phospholipid efflux; phospholipid homeostasis; phospholipid translocation; platelet dense granule organization and biogenesis; positive regulation of cAMP biosynthetic process; protein amino acid lipidation; regulation of Cdc42 protein signal transduction; response to drug; response to low density lipoprotein stimulus; response to nutrient; reverse cholesterol transport

Disease: Hypercholesterolemia, Familial; Hypoalphalipoproteinemia, Primary; Tangier Disease