Rat Complement 1 Inhibitor ELISA Kit | C1INH elisa kit

Rat Complement 1 Inhibitor ELISA Kit

Cat. Num. AAA763906

• Gene Names: SERPING1; C1IN; C1NH; HAE1; HAE2; C1INH
• Reactivity: Rat
• Synonyms: Complement 1 Inhibitor; Rat Complement 1 Inhibitor ELISA Kit; C1INH/SERPING1/C1IN/C1INH/C1NH/HAE1/HAE2/C1 Inhibitor/C1IN/C1INH/C1NH/HAE1/HAE2/SERPING1 serpin peptidase inhibitor; clade G (C1 inhibitor); member 1/Plasma protease C1 inhibitor/Serpin G1/C1 esterase inhibitor/C1-inhibiting factor; C1INH elisa kit

• Reactivity: Rat
• Sequence Length: 111

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 7.813-500ng/ml
• Sensitivity: 4.688ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for C1INH elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat C1INH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat C1INH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat C1INH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat C1INH can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 33465583
• NCBI GeneID: 710
• Molecular Weight: 55,769 Da
• NCBI Official Full Name: C1 inhibitor mutant, partial
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
• NCBI Official Symbol: SERPING1
• NCBI Official Synonym Symbols: C1IN; C1NH; HAE1; HAE2; C1INH
• NCBI Protein Information: plasma protease C1 inhibitor
• UniProt Protein Name: Plasma protease C1 inhibitor
• UniProt Gene Name: SERPING1
• UniProt Synonym Gene Names: C1IN; C1NH; C1 Inh; C1Inh
• UniProt Entry Name: IC1_HUMAN

NCBI Description

This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPING1: a protein protease inhibitor (C1-inhibitor) that forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play an important role in regulating complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Mutations of the SERPING1 gene is associated with adult macular degeneration can also cause hereditary angioedema. Binds to E.coli stcE which allows localization of SERPING1 to cell membranes thus protecting the bacteria against complement-mediated lysis. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: extracellular region; extracellular space

Molecular Function: protein binding; serine-type endopeptidase inhibitor activity

Biological Process: aging; blood circulation; blood coagulation; blood coagulation, intrinsic pathway; complement activation, classical pathway; fibrinolysis; innate immune response; negative regulation of complement activation, lectin pathway; platelet activation; platelet degranulation

Disease: Angioedema, Hereditary, Type I; Complement Component 4, Partial Deficiency Of