Human Uromodulin ELISA Kit | UMOD elisa kit

Human Uromodulin ELISA Kit

Cat. Num. AAA763831

• Gene Names: UMOD; THP; FJHN; HNFJ; THGP; HNFJ1; MCKD2; ADMCKD2
• Reactivity: Human
• Synonyms: Uromodulin; Human Uromodulin ELISA Kit; UMOD/ADMCKD2/FJHN/HNFJ/MCKD2/THGP/THP/UMOD/Uromucoid/ADMCKD2/FJHN/HNFJ/HNFJ1/MCKD2/Tamm-Horsfall glycoprotein/THGP/THP/uromodulin (uromucoid; Tamm-Horsfall glycoprotein)/uromucoid/Tamm-Horsfall urinary glycoprotein (THP)/ADMCKD2/FJHN/HNFJ/HNFJ1/MCKD2/THGP/THP; UMOD elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of UMOD. No significant cross-reactivity or interference between UMOD and analogues was observed.
• Sequence Length: 640

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UMOD elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 59850812
• NCBI GeneID: 7369
• NCBI Accession #: NP_003352.2
• NCBI GenBank Nucleotide #: NM_003361.3
• Molecular Weight: 73,571 Da
• NCBI Official Full Name: uromodulin isoform a preproprotein
• NCBI Official Synonym Full Names: uromodulin
• NCBI Official Symbol: UMOD
• NCBI Official Synonym Symbols: THP; FJHN; HNFJ; THGP; HNFJ1; MCKD2; ADMCKD2
• NCBI Protein Information: uromodulin
• UniProt Protein Name: Uromodulin
• Protein Family: Uromodulin
• UniProt Gene Name: UMOD
• UniProt Synonym Gene Names: THP
• UniProt Entry Name: UROM_HUMAN

NCBI Description

The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

UMOD: Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure providing the water barrier permeability. May serve as a receptor for binding and endocytosis for cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelial. Defects in UMOD are the cause of familial juvenile hyperuricemic nephropathy type 1 (HNFJ1). HNFJ1 is a renal disease characterized by juvenil onset of hyperuricemia, polyuria, progressive renal failure, and gout. The disease is associated with interstitial pathological changes resulting in fibrosis. Defects in UMOD are the cause of medullary cystic kidney disease type 2 (MCKD2). MCKD2 is a form of tubulointerstitial nephropathy characterized by formation of renal cysts at the corticomedullary junction. It is characterized by adult onset of impaired renal function and salt wasting resulting in end-stage renal failure by the sixth decade. Defects in UMOD are the cause of glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI). GCKDHI is a renal disorder characterized by a cystic dilation of Bowman space, a collapse of glomerular tuft, and hyperuricemia due to low fractional excretion of uric acid and severe impairment of urine concentrating ability. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 16p12.3

Cellular Component: apical plasma membrane; basolateral plasma membrane; cytoplasmic vesicle; extracellular space; extrinsic to membrane; Golgi apparatus; lipid raft; spindle pole

Molecular Function: calcium ion binding; IgG binding

Biological Process: cellular defense response; chemical homeostasis; excretion; heterophilic cell adhesion; leukocyte adhesion; negative regulation of cell proliferation; response to organic substance

Disease: Glomerulocystic Kidney Disease With Hyperuricemia And Isosthenuria; Hyperuricemic Nephropathy, Familial Juvenile, 1; Medullary Cystic Kidney Disease 2