Mouse Slit homolog 2 protein ELISA Kit | Slit2 elisa kit

Mouse Slit homolog 2 protein ELISA Kit

Cat. Num. AAA763653

• Gene Names: SLIT2; SLIL3; Slit-2
• Reactivity: Mouse
• Synonyms: Slit homolog 2 protein; Mouse Slit homolog 2 protein ELISA Kit; Slit2 (Slit Homolog 2)/SLIT2/SLIL3/slit (Drosophila) homolog 2/slit homolog 2 (Drosophila)/slit homolog 2 protein/Slit-2SLIL3; Slit2 elisa kit

• Reactivity: Mouse
• Sequence Length: 1529

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Slit2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Slit2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Slit2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Slit2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMouse Slit2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4759146
• NCBI GeneID: 9353
• NCBI Accession #: NP_004778.1
• NCBI GenBank Nucleotide #: NM_004787.3
• Molecular Weight: 168,893 Da
• NCBI Official Full Name: slit homolog 2 protein isoform 1
• NCBI Official Synonym Full Names: slit guidance ligand 2
• NCBI Official Symbol: SLIT2
• NCBI Official Synonym Symbols: SLIL3; Slit-2
• NCBI Protein Information: slit homolog 2 protein
• UniProt Protein Name: Slit homolog 2 protein
• Protein Family: Slit homolog 2 protein
• UniProt Gene Name: SLIT2
• UniProt Synonym Gene Names: SLIL3; Slit-2
• UniProt Entry Name: SLIT2_HUMAN

NCBI Description

This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

SLIT2: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth- stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post- crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; membrane; plasma membrane; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; chemorepellent activity; GTPase inhibitor activity; heparan sulfate proteoglycan binding; heparin binding; identical protein binding; laminin-1 binding; protein binding; protein homodimerization activity; proteoglycan binding; Roundabout binding

Biological Process: axon extension involved in axon guidance; axon guidance; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; cell-cell adhesion; cellular response to hormone stimulus; chemorepulsion involved in embryonic olfactory bulb interneuron migration; chemorepulsion involved in postnatal olfactory bulb interneuron migration; corticospinal neuron axon guidance through the spinal cord; dorsal/ventral axon guidance; in utero embryonic development; induction of negative chemotaxis; metanephros development; motor axon guidance; negative chemotaxis; negative regulation of actin filament polymerization; negative regulation of axon extension; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of inflammatory response; negative regulation of leukocyte chemotaxis; negative regulation of protein amino acid phosphorylation; negative regulation of small GTPase mediated signal transduction; negative regulation of smooth muscle cell migration; negative regulation of vascular permeability; positive regulation of apoptosis; positive regulation of axonogenesis; response to cortisol stimulus; response to nutrient levels; retinal ganglion cell axon guidance; spinal cord development; ureteric bud development