Aminolevulinate Delta Dehydratase ELISA Kit | Alad elisa kit

Rat Aminolevulinate Delta Dehydratase ELISA Kit

Cat. Num. AAA763024

• Gene Names: ALAD; PBGS; ALADH
• Reactivity: Rat
• Synonyms: Aminolevulinate Delta Dehydratase; Rat Aminolevulinate Delta Dehydratase ELISA Kit; Alad/ALADH/Porphobilinogen synthase; Alad elisa kit

• Reactivity: Rat
• Sequence Length: 339

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Alad elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Alad antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Alad antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Alad amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Alad can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119607786
• NCBI GeneID: 210
• Molecular Weight: 39,034 Da
• NCBI Official Full Name: aminolevulinate, delta-, dehydratase, isoform CRA_a
• NCBI Official Synonym Full Names: aminolevulinate dehydratase
• NCBI Official Symbol: ALAD
• NCBI Official Synonym Symbols: PBGS; ALADH
• NCBI Protein Information: delta-aminolevulinic acid dehydratase
• UniProt Protein Name: Delta-aminolevulinic acid dehydratase
• UniProt Gene Name: ALAD
• UniProt Synonym Gene Names: ALADH
• UniProt Entry Name: HEM2_HUMAN

NCBI Description

The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

ALAD: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Defects in ALAD are the cause of acute hepatic porphyria (AHEPP). A form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AHP is characterized by attacks of gastrointestinal disturbances, abdominal colic, paralysis, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors. Belongs to the ALADH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.2.1.24; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Lyase

Chromosomal Location of Human Ortholog: 9q33.1

Cellular Component: cytosol; extracellular space; nucleus

Molecular Function: catalytic activity; identical protein binding; lead ion binding; porphobilinogen synthase activity; zinc ion binding

Biological Process: heme biosynthetic process; porphyrin metabolic process; protein homooligomerization; protoporphyrinogen IX biosynthetic process; response to activity; response to aluminum ion; response to amino acid stimulus; response to arsenic; response to cadmium ion; response to cobalt ion; response to drug; response to ethanol; response to glucocorticoid stimulus; response to herbicide; response to hypoxia; response to ionizing radiation; response to iron ion; response to lipopolysaccharide; response to mercury ion; response to methylmercury; response to oxidative stress; response to selenium ion; response to vitamin B1; response to vitamin E; response to zinc ion

Disease: Porphyria, Acute Hepatic