Human Polyubiquitin-B ELISA Kit | UBB elisa kit

Human Polyubiquitin-B ELISA Kit

Cat. Num. AAA762945

• Reactivity: Human
• Synonyms: Polyubiquitin-B; Human Polyubiquitin-B ELISA Kit; UBB/polyubiquitin B/polyubiquitin-B/RPS27A/UBA52/UBC/ubiquitin B/Ubiquitin+1; UBB elisa kit

• Reactivity: Human
• Sequence Length: 305

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for UBB elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human UBBantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human UBB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human UBBamount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman UBBcan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 768711624
• NCBI GeneID: 192255
• NCBI Accession #: NP_620250.2
• NCBI GenBank Nucleotide #: NM_138895.2
• Molecular Weight: 34,369 Da
• NCBI Official Full Name: polyubiquitin-B
• NCBI Official Synonym Full Names: ubiquitin B
• NCBI Official Symbol: Ubb
• NCBI Protein Information: polyubiquitin-B
• UniProt Protein Name: Polyubiquitin-B
• Protein Family: Polyubiquitin
• UniProt Gene Name: Ubb
• UniProt Entry Name: UBB_RAT

NCBI Description

mediates ATP-dependent degradation of denatured proteins [RGD, Feb 2006]

Uniprot Description

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.