Mouse Microtubule-associated proteins 1A/1B light chain 3B ELISA Kit | Map1lc3b elisa kit

Mouse Microtubule-associated proteins 1A/1B light chain 3B ELISA Kit

Cat. Num. AAA762912

• Reactivity: Mouse
• Synonyms: Microtubule-associated proteins 1A/1B light chain 3B; Mouse Microtubule-associated proteins 1A/1B light chain 3B ELISA Kit; Map1lc3b/Microtubule-associated protein 1 light chain 3 beta/MAP1A/MAP1B light chain 3 B (MAP1A/MAP1B LC3 B)/MAP1 light chain 3-like protein 2/Autophagy-related protein LC3 B/Autophagy-related ubiquitin-like modifier LC3 B; Map1lc3b elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Map1lc3b. No significant cross-reactivity or interference between Map1lc3b and analogues was observed.
• Sequence Length: 125

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 78-5000pg/ml
• Sensitivity: <46.9pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Map1lc3b were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Map1lc3b were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Map1lc3b elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Map1lc3b antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Map1lc3b antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Map1lc3b amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Map1lc3b can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 47564106
• NCBI GeneID: 408001
• NCBI Accession #: NP_001001169.1
• NCBI GenBank Nucleotide #: NM_001001169.1
• Molecular Weight: 14,704 Da
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3B
• NCBI Official Symbol: MAP1LC3B
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3B
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3B
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3B
• UniProt Synonym Gene Names: MAP1ALC3; MAP1LC3; MAP1A/MAP1B LC3 B
• UniProt Entry Name: MLP3B_BOVIN

Uniprot Description

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.