Mouse Insulin-like growth factor-binding protein 7 ELISA Kit | IGFBP-7 elisa kit

Mouse Insulin-like growth factor-binding protein 7 ELISA Kit

Cat. Num. AAA762872

• Gene Names: IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
• Reactivity: Mouse
• Synonyms: Insulin-like growth factor-binding protein 7; Mouse Insulin-like growth factor-binding protein 7 ELISA Kit; IGFBP-7/AGM/FSTL2/IBP-7/IGFBP7/IGFBP-7v/IGFBPRP1/MAC25/PSF/RAMSVPS/TAF; IGFBP-7 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of IGFBP-7. No significant cross-reactivity or interference between IGFBP-7 and analogues was observed.
• Sequence Length: 282

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IGFBP-7 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IGFBP-7 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP-7 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IGFBP-7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IGFBP-7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGFBP-7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IGFBP-7 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504619
• NCBI GeneID: 3490
• NCBI Accession #: NP_001544.1
• NCBI GenBank Nucleotide #: NM_001553.2
• Molecular Weight: 28,860 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 7 isoform 1
• NCBI Official Synonym Full Names: insulin like growth factor binding protein 7
• NCBI Official Symbol: IGFBP7
• NCBI Official Synonym Symbols: AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
• NCBI Protein Information: insulin-like growth factor-binding protein 7
• UniProt Protein Name: Insulin-like growth factor-binding protein 7
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP7
• UniProt Synonym Gene Names: MAC25; PSF; IBP-7; IGF-binding protein 7; IGFBP-7; TAF
• UniProt Entry Name: IBP7_HUMAN

NCBI Description

This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011]

Uniprot Description

IGFBP7: Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. Defects in IGFBP7 are the cause of retinal arterial macroaneurysm with supravalvular pulmonic stenosis (RAMSVPS). RAMSVPS is an autosomal recessive condition characterized by the bilateral appearance of 'beading' along the major retinal arterial trunks, with the subsequent formation of macroaneurysms. Affected individuals also have supravalvular pulmonic stenosis, often requiring surgical correction.

Protein type: Cell cycle regulation; Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: insulin-like growth factor binding; protein binding

Biological Process: cell adhesion; inner ear development; negative regulation of cell proliferation; regulation of cell growth

Disease: Retinal Arterial Macroaneurysm With Supravalvular Pulmonic Stenosis