Mouse Tartrate-resistant acid phosphatase type 5 ELISA Kit | Acp5 elisa kit

Mouse Tartrate-resistant acid phosphatase type 5 ELISA Kit

Cat. Num. AAA762718

• Gene Names: ACP5; HPAP; TRAP; SPENCDI
• Reactivity: Mouse
• Synonyms: Tartrate-resistant acid phosphatase type 5; Mouse Tartrate-resistant acid phosphatase type 5 ELISA Kit; Acp5/T5ap/Trap/Tartrate-resistant acid phosphatase type 5/TR-AP/Tartrate-resistant acid ATPase/TrATPase/Type 5 acid phosphatase; Acp5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Acp5. No significant cross-reactivity or interference between Acp5 and analogues was observed.
• Sequence Length: 325

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20mIU/ml
• Sensitivity: 0.188mIU/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Acp5 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 161377455
• NCBI GeneID: 54
• NCBI Accession #: NP_001104506.1
• NCBI GenBank Nucleotide #: NM_001111036.1
• Molecular Weight: 36,599 Da
• NCBI Official Full Name: tartrate-resistant acid phosphatase type 5
• NCBI Official Synonym Full Names: acid phosphatase 5, tartrate resistant
• NCBI Official Symbol: ACP5
• NCBI Official Synonym Symbols: HPAP; TRAP; SPENCDI
• NCBI Protein Information: tartrate-resistant acid phosphatase type 5
• UniProt Protein Name: Tartrate-resistant acid phosphatase type 5
• Protein Family: Tartrate-resistant acid phosphatase
• UniProt Gene Name: ACP5
• UniProt Synonym Gene Names: TR-AP; TrATPase
• UniProt Entry Name: PPA5_HUMAN

NCBI Description

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]

Uniprot Description

ACP5: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI). A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Protein type: EC 3.1.3.2; Phosphatase; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - riboflavin

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytosol; extracellular space; integral to membrane; lysosome

Molecular Function: acid phosphatase activity; ferric iron binding; ferrous iron binding

Biological Process: bone resorption; defense response to Gram-positive bacterium; dephosphorylation; multicellular organismal response to stress; negative regulation of cell adhesion; negative regulation of inflammatory response; negative regulation of interleukin-1 beta production; negative regulation of interleukin-12 production; negative regulation of nitric oxide biosynthetic process; negative regulation of superoxide release; negative regulation of tumor necrosis factor production; ossification; osteoclast differentiation; positive regulation of cell migration; response to cytokine stimulus; response to L-ascorbic acid; response to lipopolysaccharide; response to zinc ion; riboflavin metabolic process; vitamin metabolic process; water-soluble vitamin metabolic process

Disease: Spondyloenchondrodysplasia With Immune Dysregulation