Human Forkhead box protein O3 ELISA Kit | FOXO3 elisa kit

Human Forkhead box protein O3 ELISA Kit

Cat. Num. AAA762485

• Gene Names: FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• Reactivity: Human
• Synonyms: Forkhead box protein O3; Human Forkhead box protein O3 ELISA Kit; FOXO3 (Forkhead box protein O3)/AF6q21/FKHRL1/FKHRL1P2/FOXO3A/forkhead box O3/Forkhead in rhabdomyosarcoma-like 1; FOXO3 elisa kit

• Reactivity: Human
• Sequence Length: 673

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FOXO3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human FOXO3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human FOXO3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FOXO3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human FOXO3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 42519916
• NCBI GeneID: 2309
• NCBI Accession #: NP_963853.1
• NCBI GenBank Nucleotide #: NM_201559.2
• Molecular Weight: 48,406 Da
• NCBI Official Full Name: forkhead box protein O3
• NCBI Official Synonym Full Names: forkhead box O3
• NCBI Official Symbol: FOXO3
• NCBI Official Synonym Symbols: FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• NCBI Protein Information: forkhead box protein O3
• UniProt Protein Name: Forkhead box protein O3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO3
• UniProt Entry Name: FOXO3_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.

Protein type: Oncoprotein; Autophagy; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm; nucleus

Molecular Function: beta-catenin binding; chromatin DNA binding; DNA binding; protein binding; protein kinase binding; sequence-specific DNA binding; transcription factor activity

Biological Process: anatomical structure morphogenesis; antral ovarian follicle growth; cell differentiation; DNA damage response, signal transduction by p53 class mediator; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; glucose homeostasis; initiation of primordial ovarian follicle growth; innate immune response; negative regulation of transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; oocyte maturation; ovulation from ovarian follicle; phosphoinositide-mediated signaling; positive regulation of apoptosis; positive regulation of erythrocyte differentiation; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; regulation of translation; transcription from RNA polymerase II promoter; tumor necrosis factor-mediated signaling pathway