Human Semaphorin-5A ELISA Kit | SEMA5A elisa kit

Human Semaphorin-5A ELISA Kit

Cat. Num. AAA762007

• Gene Names: SEMA5A; semF; SEMAF
• Reactivity: Human
• Synonyms: Semaphorin-5A; Human Semaphorin-5A ELISA Kit; SEMA5A/Semaphorin-5A/Semaphorin-F/Sema F/SEMAF/semaphorin F/SEMF/transmembrane domain (TM) and short cytoplasmic domain; (semaphorin) 5A; SEMA5A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SEMA5A. No significant cross-reactivity or interference between SEMA5A and analogues was observed.
• Sequence Length: 1074

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA5A elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 147904700
• NCBI GeneID: 9037
• NCBI Accession #: NP_003957.2
• NCBI GenBank Nucleotide #: NM_003966.2
• Molecular Weight: 120,615 Da
• NCBI Official Full Name: semaphorin-5A
• NCBI Official Synonym Full Names: semaphorin 5A
• NCBI Official Symbol: SEMA5A
• NCBI Official Synonym Symbols: semF; SEMAF
• NCBI Protein Information: semaphorin-5A
• UniProt Protein Name: Semaphorin-5A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA5A
• UniProt Synonym Gene Names: SEMAF; Sema F
• UniProt Entry Name: SEM5A_HUMAN

NCBI Description

This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]

Uniprot Description

SEMA5A: a single-pass type I membrane protein that may act as positive axonal guidance cues.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: integral to membrane; membrane; plasma membrane

Molecular Function: axon guidance receptor activity; chemorepellent activity; heparan sulfate proteoglycan binding; semaphorin receptor binding; syndecan binding

Biological Process: axon extension; axon guidance; axonal fasciculation; blood vessel endothelial cell proliferation during sprouting angiogenesis; cell adhesion; cell-cell signaling; detection of light stimulus involved in visual perception; diencephalon development; negative chemotaxis; negative regulation of axon extension involved in axon guidance; negative regulation of cell adhesion; nervous system development; neural crest cell migration; patterning of blood vessels; positive chemotaxis; positive regulation of actin filament depolymerization; positive regulation of angiogenesis; positive regulation of axon extension involved in axon guidance; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of protein kinase B signaling cascade