Human Interleukin-37 ELISA Kit | IL37 elisa kit

Human Interleukin-37 ELISA Kit

Cat. Num. AAA761932

• Gene Names: IL37; FIL1; FIL1Z; IL-1H; IL-37; IL1F7; IL1H4; IL-1F7; IL-1H4; IL1RP1; IL-1RP1; FIL1(ZETA)
• Reactivity: Human
• Synonyms: Interleukin-37; Human Interleukin-37 ELISA Kit; IL37 (Interleukin-37)/FIL1 zeta/IL-1F7/FIL1/FIL1 (ZETA)/FIL1Z/IL1H4/IL1F7/IL-1H4/IL-1F7b/IL-1H/IL-1RP1/IL-1X protein/IL1RP1/interleukin 1 family member 7/IL-1X/interleukin 1 family; member 7 (zeta)/interleukin 1; zeta/interleukin-1 family member 7/Interleukin-1 homolog 4/interleukin-1 superfamily z/Interleukin-1 zeta/Interleukin-1-related protein; IL37 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Agt. No significant cross-reactivity or interference between Agt and analogues was observed.
• Sequence Length: 218

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL37 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20127524
• NCBI GeneID: 27178
• NCBI Accession #: NP_055254.2
• NCBI GenBank Nucleotide #: NM_014439.3
• Molecular Weight: 17,459 Da
• NCBI Official Full Name: interleukin-37 isoform 1
• NCBI Official Synonym Full Names: interleukin 37
• NCBI Official Symbol: IL37
• NCBI Official Synonym Symbols: FIL1; FIL1Z; IL-1H; IL-37; IL1F7; IL1H4; IL-1F7; IL-1H4; IL1RP1; IL-1RP1; FIL1(ZETA)
• NCBI Protein Information: interleukin-37
• UniProt Protein Name: Interleukin-37
• Protein Family: Interleukin
• UniProt Gene Name: IL37
• UniProt Synonym Gene Names: FIL1Z; IL1F7; IL1H4; IL1RP1; IL-1F7; IL-1H; IL-1H4; IL-1 zeta; IL-1RP1; IL-37
• UniProt Entry Name: IL37_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). This cytokine also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18. This gene along with eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL37: Suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Suppresses, or reduces, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation. Belongs to the IL-1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q12-q14.1

Cellular Component: cytoplasm; cytosol; extracellular region; extracellular space; nucleolus; nucleus

Molecular Function: cytokine activity; interleukin-1 receptor binding

Biological Process: cytokine and chemokine mediated signaling pathway; immune response; inflammatory response to antigenic stimulus