Rat Perforin-1 ELISA Kit | Prf1 elisa kit

Rat Perforin-1 ELISA Kit

Cat. Num. AAA761861

• Gene Names: PRF1; P1; PFP; FLH2; PFN1; HPLH2
• Reactivity: Rat
• Synonyms: Perforin-1; Rat Perforin-1 ELISA Kit; Prf1; Prf1 elisa kit

• Reactivity: Rat
• Sequence Length: 555

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Prf1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Prf1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Prf1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Prf1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Prf1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 133908621
• NCBI GeneID: 5551
• NCBI Accession #: NP_001076585.1
• NCBI GenBank Nucleotide #: NM_001083116.1
• Molecular Weight: 61,377 Da
• NCBI Official Full Name: perforin-1
• NCBI Official Synonym Full Names: perforin 1
• NCBI Official Symbol: PRF1
• NCBI Official Synonym Symbols: P1; PFP; FLH2; PFN1; HPLH2
• NCBI Protein Information: perforin-1
• UniProt Protein Name: Perforin-1
• Protein Family: Perforin
• UniProt Gene Name: PRF1
• UniProt Synonym Gene Names: PFP; P1; PFP
• UniProt Entry Name: PERF_HUMAN

NCBI Description

The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: cytoplasmic membrane-bound vesicle; extracellular region; integral to membrane; membrane; plasma membrane

Molecular Function: calcium ion binding; protein binding; wide pore channel activity

Biological Process: apoptosis; cellular defense response; cytolysis; defense response to tumor cell; defense response to virus; formation of immunological synapse; immune response to tumor cell; protein homooligomerization; transmembrane transport

Disease: Aplastic Anemia; Hemophagocytic Lymphohistiocytosis, Familial, 2; Lymphoma, Non-hodgkin, Familial