Human Big Dynorphin ELISA Kit | Dyn elisa kit

Human Big Dynorphin ELISA Kit

Cat. Num. AAA761709

• Reactivity: Human
• Synonyms: Big Dynorphin; Human Big Dynorphin ELISA Kit; Dyn/Big Dynorphin; Dyn elisa kit

• Reactivity: Human

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 7.813-500pg/ml
• Sensitivity: 4.688pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Dyn elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human Dyn. During the reaction,Human Dyn in the sample or standard competes with a fixed amount of Human Dyn on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Human Dyn. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Dyn in the samples is then determined by comparing the O.D. of the samples to the standard curve.