Human Interleukin-20 receptor subunit alpha ELISA Kit | IL20RA elisa kit

Human Interleukin-20 receptor subunit alpha ELISA Kit

Cat. Num. AAA761431

• Gene Names: IL20RA; CRF2-8; IL-20R1; IL-20RA; IL-20R-alpha
• Reactivity: Human
• Synonyms: Interleukin-20 receptor subunit alpha; Human Interleukin-20 receptor subunit alpha ELISA Kit; IL20RA/CRF2-8/zcytor7/class II cytokine receptor ZCYTOR7/Cytokine receptor class-II member 8/Cytokine receptor family 2 member 8/IL-20R1/IL-20 receptor subunit alpha/IL-20RA/IL-20R-alpha/interleukin 20 receptor; alpha/interleukin-20 receptor I/interleukin-20 receptor subunit alpha; IL20RA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL20RA. No significant cross-reactivity or interference between IL20RA and analogues was observed.
• Sequence Length: 553

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL20RA elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 524590113
• NCBI GeneID: 53832
• NCBI Accession #: NP_055247.3
• NCBI GenBank Nucleotide #: NM_014432.3
• Molecular Weight: 57,366 Da
• NCBI Official Full Name: interleukin-20 receptor subunit alpha isoform 1
• NCBI Official Synonym Full Names: interleukin 20 receptor subunit alpha
• NCBI Official Symbol: IL20RA
• NCBI Official Synonym Symbols: CRF2-8; IL-20R1; IL-20RA; IL-20R-alpha
• NCBI Protein Information: interleukin-20 receptor subunit alpha
• UniProt Protein Name: Interleukin-20 receptor subunit alpha
• Protein Family: Interleukin-20 receptor
• UniProt Gene Name: IL20RA
• UniProt Synonym Gene Names: IL-20 receptor subunit alpha; IL-20R-alpha; IL-20RA; CRF2-8
• UniProt Entry Name: I20RA_HUMAN

NCBI Description

This gene encodes a member of the type II cytokine receptor family. The encoded protein is a subunit of the receptor for interleukin 20, a cytokine that may be involved in epidermal function. The interleukin 20 receptor is a heterodimeric complex consisting of the encoded protein and interleukin 20 receptor beta. This gene and interleukin 20 receptor beta are highly expressed in skin, and are upregulated in psoriasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

IL20RA: The IL20RA/IL20RB dimer is a receptor for IL19, IL20 and IL24. The IL20RA/IL10RB dimer is a receptor for IL26. Belongs to the type II cytokine receptor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q23.3

Cellular Component: integral to membrane; plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-20 binding

Biological Process: cytokine and chemokine mediated signaling pathway; regulation of bone resorption