Human Chemokine XC receptor 1 ELISA Kit | XCR1 elisa kit

Human Chemokine XC receptor 1 ELISA Kit

Cat. Num. AAA761282

• Gene Names: XCR1; GPR5; CCXCR1
• Reactivity: Human
• Synonyms: Chemokine XC receptor 1; Human Chemokine XC receptor 1 ELISA Kit; XCR1 (XC-Chemokine Receptor 1)/CCXCR1/GPR5/CCXCR1/chemokine XC receptor 1/G protein-coupled receptor 5/G-protein coupled receptor 5/Lymphotactin receptor; XCR1 elisa kit

• Reactivity: Human
• Sequence Length: 333

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for XCR1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- XCR1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- XCR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the XCR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of XCR1can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 66932941
• NCBI GeneID: 2829
• NCBI Accession #: NP_001019815.1
• NCBI GenBank Nucleotide #: NM_001024644.1
• Molecular Weight: 38,508 Da
• NCBI Official Full Name: chemokine XC receptor 1
• NCBI Official Synonym Full Names: chemokine (C motif) receptor 1
• NCBI Official Symbol: XCR1
• NCBI Official Synonym Symbols: GPR5; CCXCR1
• NCBI Protein Information: chemokine XC receptor 1
• UniProt Protein Name: Chemokine XC receptor 1
• Protein Family: Chemokine XC receptor
• UniProt Gene Name: XCR1
• UniProt Synonym Gene Names: CCXCR1; GPR5
• UniProt Entry Name: XCR1_HUMAN

NCBI Description

The protein encoded by this gene is a chemokine receptor belonging to the G protein-coupled receptor superfamily. The family members are characterized by the presence of 7 transmembrane domains and numerous conserved amino acids. This receptor is most closely related to RBS11 and the MIP1-alpha/RANTES receptor. It transduces a signal by increasing the intracellular calcium ions level. The viral macrophage inflammatory protein-II is an antagonist of this receptor and blocks signaling. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

XCR1: Receptor for chemokines SCYC1 and SCYC2. Subsequently transduces a signal by increasing the intracellular calcium ions level. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cytosol; integral to plasma membrane; plasma membrane

Molecular Function: chemokine receptor activity

Biological Process: chemotaxis; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; inflammatory response; release of sequestered calcium ion into cytosol