Human A-kinase anchor protein 17A ELISA Kit | AKAP17A elisa kit

Human A-kinase anchor protein 17A ELISA Kit

Cat. Num. AAA761202

• Gene Names: AKAP17A; XE7; 721P; XE7Y; CCDC133; CXYorf3; PRKA17A; SFRS17A; AKAP-17A; DXYS155E
• Reactivity: Human
• Synonyms: A-kinase anchor protein 17A; Human A-kinase anchor protein 17A ELISA Kit; AKAP17A/A-kinase anchor protein 17A/AKAP-17A/Splicing factor; arginine/serine-rich 17A/Protein kinase A-anchoring protein 17A/Protein XE7/721P/B-lymphocyte antigen; AKAP17A elisa kit

• Reactivity: Human
• Sequence Length: 695

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for AKAP17A elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human AKAP17A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human AKAP17A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human AKAP17A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human AKAP17A can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 41152097
• NCBI GeneID: 8227
• NCBI Accession #: NP_005079.2
• NCBI GenBank Nucleotide #: NM_005088.2
• Molecular Weight: 51,409 Da
• NCBI Official Full Name: A-kinase anchor protein 17A isoform 1
• NCBI Official Synonym Full Names: A-kinase anchoring protein 17A
• NCBI Official Symbol: AKAP17A
• NCBI Official Synonym Symbols: XE7; 721P; XE7Y; CCDC133; CXYorf3; PRKA17A; SFRS17A; AKAP-17A; DXYS155E
• NCBI Protein Information: A-kinase anchor protein 17A
• UniProt Protein Name: A-kinase anchor protein 17A
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP17A
• UniProt Synonym Gene Names: CXYorf3; DXYS155E; SFRS17A; XE7; AKAP-17A; PRKA17A
• UniProt Entry Name: AK17A_HUMAN

NCBI Description

This locus encodes a protein kinase A anchoring protein. The encoded protein is part of the spliceosome complex and is involved in the regulation of alternate splicing in some mRNA precursors. Alternatively spliced transcript variants have been identified for this gene.[provided by RefSeq, Sep 2010]

Uniprot Description

SFRS17A: Splice factor regulating alternative splice site selection for certain mRNA precursors. Mediates regulation of pre- mRNA splicing in a PKA-dependent manner. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing

Chromosomal Location of Human Ortholog: Xp22.33 and Yp11.32

Cellular Component: cytoplasm; nuclear speck; nucleoplasm; nucleus; spliceosome

Molecular Function: nucleotide binding; protein binding; protein kinase A binding

Biological Process: B cell activation; mRNA processing; regulation of RNA splicing; regulation of transcription, DNA-dependent; RNA splicing; signal transduction