Rabbit anti-Human CHM Polyclonal Antibody | anti-CHM antibody

anti-CHM antibody

Cat. Num. AAA7611854

• Gene Names: CHM; TCD; GGTA; REP-1; DXS540; HSD-32
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE. Immunogen affinity purified
• Synonyms: CHM; Polyclonal Antibody; anti-CHM antibody; DXS540; GGTA; HSD-32; REP-1; TCD; RAE1

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE. Immunogen affinity purified
• Form/Format: Liquid. PBS with 0.02% sodium azide and 50% glycerol pH7.3

• Applicable Applications for anti-CHM antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:20-1:200
• Immunogen: Rab proteins geranylgeranyltransferase component A 1
• Preparation and Storage: Store at -20 degree C for 12 months. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7611854(CHM antibody) at dilution of 1:100)

Western Blot (WB)

(HEK293 cells were subjected to SDS PAGE followed by western blot with MBS7611854(CHM antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 119618965
• NCBI GeneID: 1121
• UniProt Accession #: P24386; O43732; A1L4D2
• Molecular Weight: 12,301 Da
• NCBI Official Full Name: choroideremia (Rab escort protein 1)
• NCBI Official Synonym Full Names: CHM, Rab escort protein 1
• NCBI Official Symbol: CHM
• NCBI Official Synonym Symbols: TCD; GGTA; REP-1; DXS540; HSD-32
• NCBI Protein Information: rab proteins geranylgeranyltransferase component A 1
• UniProt Protein Name: Rab proteins geranylgeranyltransferase component A 1
• Protein Family: Rab proteins geranylgeranyltransferase component
• UniProt Gene Name: CHM
• UniProt Synonym Gene Names: REP1; TCD; REP-1

NCBI Description

This gene encodes component A of the RAB geranylgeranyl transferase holoenzyme. In the dimeric holoenzyme, this subunit binds unprenylated Rab GTPases and then presents them to the catalytic Rab GGTase subunit for the geranylgeranyl transfer reaction. Rab GTPases need to be geranylgeranyled on either one or two cysteine residues in their C-terminus to localize to the correct intracellular membrane. Mutations in this gene are a cause of choroideremia; also known as tapetochoroidal dystrophy (TCD). This X-linked disease is characterized by progressive dystrophy of the choroid, retinal pigment epithelium and retina. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016]

Uniprot Description

Substrate-binding subunit of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B composed of RABGGTA and RABGGTB, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Besides, a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation.